I need to evaluate how much of an administered compound is present in mice adipose tissue. Does anyone have a protocol or paper that they can recommend on how to extract a compound from adipose tissue for LCMS-MS analysis?
i presume by extract from fat you mean you want to get the compound in pure form ? if that's the case then a simple silica gel chromatography would do the trick.
Actually, I need to extract it from adipose tissue that is fresh frozen. I will edit the original question to avoid confusion.
Extraction is generally depends upon the polarity/logP of the analyte of interest. In general Bligh Dyer (Chloroform-methanol) extraction will help.
This paper may help.
http://www.sciencedirect.com/science/article/pii/S0379073800001468?via%3Dihub
Thank you both Amjad Alkhdhairawi and Abdul Basit. I found the following paper Article A rapid, environmentally friendly, and reliable method for p...
and will give their method a try.
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts