How can I model a prediction of a dependent variable using BOTH a predictor at time 1, time 2 AND the change (time 2-1) of this predictor?

I want to model the following: DV: Y at time 3 IVs: X at time 1, X at time 2, change of X from time 1 to time 2 Which is the most appropriate way (preferably in SPSS). I am afraid I cannot use...

10 December 2020 264 3 View

Can you recommend me tree databases?

Hi. I have slight experience in searching phylogenetic trees, normaly I use TreeBASE or Dryad but some papers that I found doesn't have the resulting trees in these databases or there are genetic...

26 October 2020 582 3 View

Could ANOVAs be used as a substitute for regression models for mediation analysis?

Hello all, I hope you can help me with these questions. I am developing a mediation analysis using the mediation package in R. I need to analyze the influence of perceived learning (Mediating...

18 August 2020 7,521 9 View

What would be the fate of ecotoxicology in the South American studies?

I'm currently working on ecotoxicology and I'd like to know what my colleagues from the same area think, especially in South America. Too much of microplastics in my opinion, but few studies...

18 May 2020 1,064 2 View

(epidemiology) how does one measure the RATE of infectivity?

I am not an epidemiologist, nor am I studying epidemiology, this is a question born of sheer curiosity due to current events. I understand (more or less) the principle behind the basic...

19 March 2020 9,458 3 View

How can I clean insect exoskeleton?

I want to remove whole soft tissues and maintain intact the exoeskeleton

10 March 2020 4,345 6 View

How would you evaluate "Interpersonal Trust" for people who are sharing a 4-seat vehicle with people they do not know?

Hi. I am conducting an experiment and I would like to understand if people would be willing to share a 4-seat vehicle with people they do not know. I have thought that the personal tendency of...

10 December 2019 7,989 3 View

How to handle output data for Taguchi DOE?

I am using a L8 (2 level, 4 Factor) DOE. I have completed the experiments and am now trying to sift through the data and start the analysis. I completed these experiments in a strictly production...

03 December 2019 1,977 5 View

PDL1 expression in PB lymphocytes by flow cytometry?

is there anyone who study PDL1expression in PB lymphocytes by flow cytometry in healthy controls and in pathological cases ( cancer) Oddly, we found that healthy controls express more PDL1 than...

02 December 2019 4,873 3 View

How can i measure health well-being ?

12 August 2019 8,050 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View