A) Is SYBR green I cell/nuclear membrane permeable? B) Does it affect down stream molecular biological assays?

02 February 2017 0 5K Report

My goal is to sort cells that fluoresce with a fluorescence conjugated anti-body, and to use SYBR or other dye as a positive control. I want to take the DNA that conjugates with the antibody into sequencing (i.e. ChIP-seq).

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Science method

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View