I'm sure someone has done this many years ago but Google did not give me much info.
Does anyone know where can I get a dialysis tube small enough to put into a regular NMR tube, which has ~5mm diameter? How can I put it into a NMR tube?
Thank you for those who have replied.
I was actually trying to do some MR imaging, and would like to try first with a NMR that has two compartments. A contains enzyme and its polymeric substrates, and B only has buffer at the beginning. The monomeric product from the enzymatic hydrolysis should eventually get into B, and I need some sort of dialysis membrane to prevent the enzyme and its polymeric substrate from diffusing into B.
Putting the dialysis bag in the NMR tube and running the experiment will cause a problem with shimming and I would not advise it. A small dialysis cassette would probably be preferable.
http://www.piercenet.com/browse.cfm?fldID=27764CB8-F5A6-4505-A66C-6148EEFB8CAE
Yes your shim will suck. If you want to dialyze take the sample out of the NMR tube.
You do not have enough liquid in an NMR tube to effectively dialyze a sample anyway. Assuming then that you want to do something else...
There are some tube devices (float a lyzer or something like that) that are rather small you might be able to get them into an 8 or 10 mm tube if you cut off the top part. They also have a counterweight at the bottom. I would cut that off as well... or at least make damn sure it is not ferromagnetic!!!!
depending on what kind of imaging you want to do, the shim may not be a major issue. we did it with dialysis tubing for this project in a 10 mm NMR tube: http://onlinelibrary.wiley.com/doi/10.1002/anie.201202481/abstract
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