Does anybody know of a group that has generated a subclone of the 67NR breast cancer line that is resistant to endocrine therapies (i.e., SERMs or SERDs?)
Hello! My professor and I are looking at a gene in yeast that might have something to do with GAL gene induction via repression of heterochromatin formation. We are not sure exactly how long it...
03 April 2023 8,626 0 View
Hi All, I have a small question regarding the random-intercept cross-lagged model (RI-CLPM). Is it possible to find out, whether a positive significant cross-lagged effect implies that a positive...
22 May 2022 6,932 0 View
As the question states, I am currently doing some revisions for a paper on personal branding for artists. Although I have found enough theory in the influencer marketing area, I am interested in...
17 April 2022 2,125 7 View
I address this dilemma to historians and anthropologists. Is it or is it not beneficial to colorize archival photographs using artificial intelligence? When I first discovered this possibility it...
06 April 2022 9,575 4 View
I have recently published papers related to this theme in collaboration with two recent Pakistani doctoral graduates - Yaar Muhammad and Rehana Kousar. I would be interested to read RG members...
06 February 2022 4,123 3 View
I need to find the article with access to the actual instrument as well.
19 October 2021 4,338 2 View
Hi all, I am conducting an ELISA assay to determine the concentration of recombinant protein in the cell culture supernatant after transfection. I have many different transfection conditions, with...
12 October 2021 4,602 3 View
I recognize that you can check each site individually, but often times, the publication fee is difficult to find. Additionally, it seems that the costs to publish even a short manuscript is...
15 September 2021 2,928 0 View
This is probably a dumb question, but what is the purpose for adding an n terminal secretion peptide to my protein insert during transfection into mammalian cells? Why would I want the protein...
31 August 2021 1,460 3 View
Hi all, I am transfecting HEK293T cells with SARS-Cov-2 spike protein. I will likely be transfecting the entire sequence after I clone my insert into my plasmid. However, most publications do not...
31 August 2021 4,641 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.
22 July 2024 9,954 4 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
19 July 2024 8,522 0 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
You have mentioned as a co-author of one publication, but it is not exactly a publication , it is an abstract submitted to: Congresso Brasileiro de Hematologia, Hemoterapia e Terapia Celular -...
17 July 2024 4,035 15 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View