I have 100 mg of 3-MA (autophagy inhibitor) powder.
I'm gonna add 1.342 mL of pre-warmed sterile water to make 0.5 M stock.
Can I do that? Will it be precipitated at that high concentration?
If not, please suggest me another solution.
Thanks for your interest in my question!
3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K).
Please see the reference given below:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665
The 3-MA has been reported to inhibit autophagy at concentrations ranging from 1-10 mM was directly dissolved into the culture medium at the indicated concentrations. The solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.
The 3-MA is dissolved in DMSO (warmed with 50ºC water bath) 10 mg/mL (67.05 mM), while in water (warmed with 50ºC water bath) 4 mg/mL (26.82 mM).
The choice is yours
Best
https://www.researchgate.net/post/Can_anyone_suggest_how_to_solubilize_the_3-Methyl_adenine-autophagy_inhibitor
https://www.invivogen.com/sites/default/files/invivogen/products/files/3_methyladenine_tds.pdf
https://www.sigmaaldrich.com/TR/en/product/sigma/m9281
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