2D protein gel system?

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Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

The algal cell always gather together when the cells concentration is high and those cells would block the microfluidic chips. How can i handle it?

I want to pump a high concentration algal cells into microfluidic chip, however those cells are always gather together forming a clusters to block the connection between Teflon tube and...

26 October 2020 5,366 2 View

What kind of small mobile robots are available on market to undertake multi-robot research?

Dear All, What kind of small mobile robots are available in market to undertake multi-robot research? Would you have anything to be recommended? Thanks! Chaomin Luo ~~~ Chaomin Luo,...

04 October 2020 5,173 5 View

Why some structural equation models are all observation variables?

Hello everyone. Recently, when I searched the literature about SEM, I found some problems that I couldn't solve. I found that the models established in some studies are all observation variables...

16 July 2020 3,279 3 View

Is there any open source code for learning in the field of data-driven robust optimization?

I can get a lot of theoretical knowledge in related fields, but I'm still confused about the implementation behind it. Unfortunately, I didn't find any examples on the Internet that I could...

29 March 2020 6,438 3 View

Why the large grain contains small grain inside ?

We currently recieved a sample from industry. It is high manganese steel (Hadfield steel) with a composition of Fe-12Mn-1.1C, after austenization treatment. We find that the grain size of this...

15 January 2020 6,624 1 View

How to read the intersection signal information in the Internet of Vehicles environment?

The existing method can be realized by short-distance traffic through the DSRC, or the signal light device can be connected to the roadside device RSU by wire. Even in the driverless field, the...

18 November 2019 6,706 1 View

Are there recommendations to buy some small mobile robots to test our multi-robot algorithms?

We would like to validate and test our multi-robot algorithms with some research-platformed mobile robot. Are there recommendations to buy some small mobile robots to test our multi-robot...

15 November 2019 5,772 3 View

How should the bone model be considered when screw drilling into bone finite element modeling?

For the establishment of bone model, besides considering the bone density, elastic modulus, poisson's ratio, yield stress and yield strain, should the damage caused by bone being cut by screws be...

09 November 2019 5,831 3 View

Why a picture captured from a linear array CCD have a sinusoidal stripe pattern?

We used linear array CCD to capture movement object pictures. When extracting information from the pictured, we were bothered by the intensive sinusoidal stripe pattern (like the files jpg). What...

06 October 2019 5,846 1 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to dissolve lyophilized silk fibroin?

Dear members of the community, I am trying to concentrate silk fibroin to 25 % (w/v). Nevertheless, i am struggling with the concentration step. I successfully achieve concentrations of 5-6 % of...

16 February 2021 1,808 1 View