2D protein gel system?

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Could you please support to refresh below speech?

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Does anyone know what might be causing the smeared bands on my western blot?

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Why is my ASO degraded quickly on agarose gel?

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Low-yield gel extraction problem?

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PCR showing no bands - Master mix and primers didn't mix?

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Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

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29 July 2024 950 4 View

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What is the cause of this aggregation just bellow 70 kDa mark?

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Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

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Yuri Mirgorod

Xiuquan Luo

I recommend a review of monomolecular protein films

http://www.chem.msu.ru/rus//vmgu/015/355.pdf

Abdelkader Dehbi

Please read this paper

Hadeia Mashaqbeh

Find this publication it explain simple 2D system in details,

https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.21116