I extracted RNA from spinal cord tissue using Trizol and following invitrogen's protocol for RNA extraction for PCR.
I had good 260/280 ratios (around 1,8-1,9), but very low 260/230 ratios (0,4-0,5).
I have problems to get new tissue and do the extraction again. Is there any protocol to purify the samples and try to obtain better 260/230 ratios?
Thanks