I extracted RNA from spinal cord tissue using Trizol and following invitrogen's protocol for RNA extraction for PCR.

I had good 260/280 ratios (around 1,8-1,9), but very low 260/230 ratios (0,4-0,5).

I have problems to get new tissue and do the extraction again. Is there any protocol to purify the samples and try to obtain better 260/230 ratios?

Thanks

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