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Questions related to Resistance
Hello, first of all, thank you to everyone who takes the time to answer this question. I’m trying to isolate RNA from macrophages. We use TRIzol for cell isolation from the plate. Then we purify...
15 April 2025 9,724 3 View
I would be grateful if anyone could share a copy of the CLSI VET guidelines from 2022 or later.
09 April 2025 653 0 View
I'm starting a systematic review which will include systematic reviews, meta-analyses, randomized controlled trials (RCTs), observational studies, qualitative research, and mixed-methods studies....
03 April 2025 5,397 3 View
Hi all, How can I perform buffer exchange and concentrate my protein after I removed my endotoxin using the Triton-X 114 method? All of the concentrators that I tried (Vivaspin and Thermo's...
28 March 2025 9,049 0 View
Hello Abishake Sir, I am working on an AI project for detecting diseases in cows and found your research on 'Mastitis-Disease-Detection' very interesting. I would like to use your dataset for my...
25 March 2025 1,706 0 View
I’m working with a T4-phage genome and obtained two different assemblies: one using a de novo strategy and the other using a reference genome. The reference-guided assembly resulted in a larger...
21 March 2025 6,326 3 View
I recently performed mouse glomerulus isolation and obtained the attached microscopy image. Before proceeding with further RNA/protein analysis, I would like to confirm whether these structures...
12 March 2025 3,510 0 View
definition of concepts is in detail must be included
11 March 2025 9,819 0 View
I attempted to superimpose two protein structures using UCSF Chimera, and the calculated RMSD was 1.33. However, the structures were not properly aligned. I also calculated RMSD using PyMOL, which...
04 March 2025 736 3 View
I am trying to express proteins in CHO DG44 cells. I am following the methods in the Freedom™ DG44 Kit USER GUIDE. After transfection, I recovered viability to 90% and adapted cells to 1 µM MTX...
26 February 2025 6,274 3 View
Why I cannot find these sequences I did that for other bacteria it worked perfectly
25 February 2025 7,665 0 View
I tryed to add an article published in ACS Omega, but ACS Omega is not in the journal list.
21 February 2025 915 3 View
We are setting up PingFederate in GCP env using devops. The vanilla Ping authentication works without issues. However, helm charts of Pingfederate does not have annotations / variables to...
21 February 2025 3,700 5 View
Hi I am doing a systematic review and completed my title and abstract screen in March 2024. After that I took some break and want to continue that work. Please guide 1. Do I need to remove all...
20 February 2025 4,815 3 View
children have been known to recognize noises early on in the developmental stage. Im wondering if you can stimulate more synapse growth by exposing the child to different auditory stimuli.
18 February 2025 1,062 0 View
Hi Researchers I am doing meta-analysis for one of my SR. Could you please from your expertise suggests if we can do the meta-analysis of RCT and multiarm-RCTs together? Thank you
16 February 2025 9,971 1 View
Hi Is it possible to combine RCT studies along with multi-arm RCT studies for conducting meta-analysis. If so, could you please send me the citation? Kind regards
15 February 2025 1,741 0 View
Joint extented Kalman filter-based parameter estimation in networked SEIR models with stochastic variability: analysis using COVID-19 daily data from southern India
08 February 2025 9,730 1 View
I'll thank you in advance for the help.
07 February 2025 7,681 0 View
Dear all, I need to perform an MD simulation of a Pt cluster on a carbon surface using the LAMMPS software. I have used the EAM potential to describe the Pt-Pt interaction. The simulation...
06 February 2025 8,436 5 View
Pls tell?
29 January 2025 8,605 4 View
Efficiency in statistical software operations through data computation, analysis, interpretation and correct reporting can help to achieve increased confidence in research results. This efficiency...
21 January 2025 6,844 3 View
Hello everyone, Could you please recommend: - High-quality, heat-resistant double-sided tape brands - Suitable glass plates Specifically used for mounting and analyzing: - Zircons - Other heavy...
17 January 2025 7,160 0 View
Hi all, I am conducing a scoping review centred on Adverse Childhood Experiences and Positive Childhood Experiences as part of my thesis. I am really struggling to identify eligible studies. I...
06 January 2025 8,701 1 View