How to perform buffer exchange / protein concentration ater endotoxin removal?

28 March 2025 0 9K Report

Hi all,

How can I perform buffer exchange and concentrate my protein after I removed my endotoxin using the Triton-X 114 method?

All of the concentrators that I tried (Vivaspin and Thermo's Pierce concentrator) have high endotoxin levels.

Thanks.

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