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Questions related to Reasoning
I have been simulating pipe flow with velocity inlet and pressure outlet system. I am using LBM. for some reason the pressure at the inlet (if we go from 1 to 2000 in length pressure at lattice...
20 July 2021 7,842 7 View
I have recorded M-H plot of BaFe12O19 at RT. Their is kink in M-H loop. what could be the possible reasons of this kink.
18 July 2021 3,801 2 View
I am using DH5 alpha recombinant cells with ampicillin resistance gene but growth of cells is very slow. I am using 50ug/ml ampicillin. what may be reason for that?
18 July 2021 9,740 3 View
In accordance with the classical limit, the probability per attempt of tunneling decreases towards zero as the mass m of a particle, the deficit V − E between its energy E and the barrier height V...
14 July 2021 3,313 3 View
Calcination typically increases the aggregation and size of nanoparticles. However, are there any exceptions? I have synthesized NiO/CuO nanoparticles. After calcination at 300 °C the size and...
14 July 2021 5,723 3 View
I have Scientific WorkPlace 5.5 from MacKichan Software. But after 40 years of providing excellent products and service, MacKichan Software is no longer in business. Are there any other companies...
14 July 2021 340 20 View
My samples are mainly: clays and clay minerals, synthetic layered double hydroxides and zeolites. Is there any reasonable alternative for Malvern Zetasizer (Advance or older NanoZS)?
13 July 2021 1,529 3 View
Zeta potential of my particles (60-80 nm ZIF-8) varies greatly with concentration. I have to optimize it for my research.
13 July 2021 2,980 3 View
Mostly research papers focus on rectangular or cylindrical fin. But on comparing them with trapezoidal fins, trapezoidal fins have more surface area. Very less papers available for trapezoidal...
09 July 2021 1,933 1 View
As a result of testing the produced fibers, an additional peak was observed in the cooling and heating diagram.
09 July 2021 1,647 3 View
I am performing nonlinear pushover analysis of RC bridge structure by defining fiber hinges and during analysis it shows, 'maximum number of null steps reached, subsequent results will not be...
06 July 2021 6,039 1 View
I reverse transcribed the RNA to the cDNA. Then I stored the cDNA in -20 freezer but after several months the volume of the cDNA decreased to 0.1. What is the reason?
06 July 2021 7,659 1 View
Can anyone help me with protocol ? Thank you in advance.
05 July 2021 1,630 1 View
please could anyone of you dears let me know which type of DEM is better for mapping flood prone areas and why with scientific reason or reference ALOS palsar dem 12.5 m and coperinose GDEM 30...
03 July 2021 497 4 View
I am performing UV/Vis spectroscopy using for pesticide effects on chlorophyll a and b. Chlorophyll a is having a tendency to bring about negative values. The absorptions are performed on three...
03 July 2021 3,956 7 View
difference?
02 July 2021 5,458 3 View
How to calculated limit of detection based on 3σ method? I read that we should calculate based on the standard deviation of the response (Sy) of the curve and the slope of the calibration curve...
02 July 2021 1,386 3 View
I used codon optimized Plasmodium falciparum apicoplast helicase gene inserted into pet28a vector system (from Genscript) transformed into Rosetta 2DE3. I did transformation for 3-4 times 1-2...
01 July 2021 7,927 3 View
I need the absorption extinction coefficient of lead chloride dissolved in DI water to calculate its concentration from my UV-VIS absorbance spectra.
01 July 2021 4,394 1 View
Hi, I am looking for reasonable linear and affine gap penalties for local pairwise alignments of DNA and amino acid sequences. I am using the NUC and BLOSUM62 substitution matrices from NCBI,...
30 June 2021 256 1 View
I have designed a few pairs of sgRNA oligos using both CHOPCHOP and CRISPOR. I checked for their alignments within themselves. However, when I did the Primer-BLAST using both oligos (excluding the...
28 June 2021 613 5 View
I'm purifying a protein using bacterial expression system. Purification is done using affinity chromatography. While on the SDS PAGE it shows band at expected mass (17 kDa) but molecular mass...
26 June 2021 7,956 2 View
We cloned a viral gene into pET28a expression vector and expressed in E. coli. Then, expressed protein was examined by WB and other methods. However, unexpectedly, molecular weight (MW~35KD) of...
24 June 2021 9,946 3 View
Botrytis cinerea infection leads to the formation of necrotrophic lesions on plant leaves. In some cases (infection with several B. cinerea strains), these lesions are surrounded by the area of...
23 June 2021 5,778 10 View