How to determine the critical denaturation temperature (Tc) during COLD-PCR assay design?

20 June 2025 0 2K Report

Is it possible to non-experimentally determine the critical denaturation temperature, by which only (or mostly) mutant DNA is melted during a fast COLD-PCR assay or a classical COLD-PCR assay? If not, how to design an assay which can help me to find the Tc, with minimal resources (minimal amount of reagents)?

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