I explored NaCl with different concentrations (0, 0.02, 0.1, and 0.5 M) on the beta-Lg hydrolysis in a flow-through monolithic reactor with immobilized trypsin. The hydrolysis efficiency was improved with the addition of NaCl up to 0.1 M NaCl, while decreased at 0.5 M. However, for the free (soluble) trypsin, at 0.1 M NaCl, a big decrease was observed.
Why?
1) The salt effects dominate differently at different concentrations
(salt bridge at low concentration vs charge shielding at high concentration)
2) The immobilized trypsin is somehow like the affinity column, just like salts (here up to 0.1 M) can promote the contact? Why? any support?
Any comments, ideas are very welcome. Thanks.
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