If I understand correctly, are you looking for suggestions for the markers used to identify these subsets?

There are a lot of differing opinions on the distinguishing markers, however these are widely accepted-

Naive T cells - CD45RA+ CCR7+ CD62L+ CD95- IL2Rbeta-

Effector T cells- CD45RO+ CD95+ IL2Rbeta+ CCR7- CD62L-

Central memory- CD45RO+ CD95+ IL2Rbeta+ CCR7+ CD62L+

Effector memory- CD45RA+ CD95+ IL2Rbeta+ CCR7- CD62L-

And ofcourse use CD4, CD8 and CD3 markers as well!

Ref- Restifo- Big bang theory of stem-like T cells confirmed (Blood 2014)

Hope this helps!

Dear Waqas,

I just briefly wanted to email you concerning your CD8 T cell question.

To assess T cell memory phenotypes in humanized mice, I would propose using antibodies against T cell lineage markers (CD3, CD4, CD8)

Naive T cells you can identify as: CD45RA+ CD45RO- CCR7+ CD27+ cells.

Central memory T cells you best identify as: CD45RA- CD45RO+ CCR7+ CD27+ CD62L+

Effector memory T cells you identify as: CD45RA- CD45RO+ CCR7- CD27- CD62L- cells

For further information, please consider the product pages of RNDSystems that provide loads of useful information and the attached review, which also gives a nice overview over the subject.

https://www.rndsystems.com/product-highlights/antibodies-memory-t-cell-subset-identification

Article The who's who of T-cell differentiation: Human memory T-cell subsets

All the best and good luck with your experiment,

Michael

Michael Förster Thanks! Nice to jump into your comment here! Good luck with whatever you are doing now :)

CD44 vs CD62L gate

CD62L lo CD44 hi = Effector Memory

CD62L hi CD44 hi = Central Memory

CD62L hi CD44 lo = Naive