How to address cholesterol efflux in primary residential macrophages? We can't access radioactivity so the classical approach couldn't be used. Any suggestions are welcome.
Thanks
Vivek
Please see this paper, might be usful.
Good day
Article Knock-down of the oxysterol receptor LXRα impairs cholestero...
You can use the fluorometric methods using BODIPY-cholesterol or NBD-cholesterol.
See these papres:
A sensitive assay for ABCA1-mediated cholesterol efflux using BODIPY-cholesterol.
The implication of cigarette smoking and cessation on macrophage cholesterol effl ux in coronary artery disease patients.
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