One S. mutans culture with us was forming good biofilm until recently. But since last few experiments, its biofilm formation is not satisfactory.
What media are you using? If you are able, you can switch to a media that promotes biofilm growth:
0.5X TY with 10mM sucrose :
For 500mL
7.5g Tryptone
2.5g Yeast
1.71g Sucrose
At the very least, try adding 10mM sucrose to your media as it helps with biofilm formation.
You can also add additional flow to the growth media to stimulate attachment ,harvest the organisms that have attached to the walls, discard the rest.
Dear M Finnegan,
Do you mean to provide shaking during incubation? OR something else?
How old is your culture? How are you sterilizing your media? We had a problem using cotton/gauze plugs years ago(I know probably very old technique), an additive in them actually killed our cultures.
If you don't care about selecting a subset of organisms, grow in the media suggested above with gentle stirring. Select the adherent organisms.
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