Timsy --

12 Questions 17 Answers 0 Followers

Questions related from Timsy --

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

27 January 2021 9,087 3 View

How can one delineate a bacterial species based on Taxonogenomic methods such as comparative genomics?

Hi all, Using polyphasic approach, I found out a novel species of bacteria. I want to delineate this species futher in the basis comparative genomics. Can you suggest some methods which can be...

10 December 2020 1,614 3 View

How to test a methylotrophic bacterial strain for its capability to use methylamine?

Hi all, I have a novel species of bacteria which has the genes/enzymes responsible for carrying out methylotrophic activity. This strain can grow on methanol as well as methylamine (on the basis...

08 December 2020 1,808 2 View

EDGAR - How to add Whole genome sequence of the strain (novel) to EDGAR comparative genomic tool for comparison?

Hi all, I am interested to do a comparative genome analysis using EDGAR comparitive genomic tool. I have a novel bacteria whose genome sequence was obtained by us and now I was to do compare this...

11 November 2020 3,879 3 View

Is it neccesary to perform API ZYM in addition to API 20NE and Biolog GEN III tests to prove a novel species of Paracoccus?

Dear all, As a requirment to prove a novel species of Paracoccus I want to conduct some physiological tests. Through literature review, I found out that some studies peformed only API 20 NE +...

17 September 2020 6,133 0 View

How to get 16srRNA gene sequence from whole genome sequence of a bacterial spp. digitally?

Hello Everyone, I have whole genome sequence of bacteria (which I want to prove as a novel spp. and 99% novel). I want to extract the 16s rRNA gene sequence from it digitally. What is the easiest...

05 May 2020 8,925 10 View

Which are the house keeping for methylotrophic bacteria?

Dear all, I have whole genome sequences of many bacteria (nitrate reducers) and I ´m interested to know if these bacteria are capable of utilizing C1- componds, Therefore, I am looking for...

15 April 2020 2,150 2 View

Based on whole genome sequence of an novel Pseudomonas species, the bacterium belongs to P.aerugionosa lineage. Can it be a potenial human pathgogen?

I have discovered a novel species of bacteria that belongs to Pseudomonas genus. On the basis of rpoD gene (individually) and 16sRNA+rpoD genes based (concatenated) trees,it shows close...

01 April 2020 2,603 6 View

How to name a novel species of Pseudomonas. What are the guidelines?

Hello everyone, I need to know the criteria to provide the species name to a novel species of Pseudomonas which I discovered during my research. Could anyone let me know the latest version of how...

03 February 2020 10,122 3 View

How to get the RpoD gene sequence of Pseudomonas T-strains to make a phylogenetic Tree?

Greetings everyone. I´m interested to get the sequence of RpoD gene for several Pseudomonas type-strains to make a phylogentic tree. I´m not sure how to get it through NCBI or if there is any...

17 October 2019 4,510 6 View

Should I use 16S RNA sequence of Type strain of bacteria to create a phylogentic tree?

I am interested to make a phylogentic tree as a requirment to prove my putative novel bacterial species. I would like to know if I should only take the Type-strain 16S RNA sequence of my related...

25 September 2019 5,746 11 View

Help : Anybody has experience with Mzmine software for metabolite identification?

Hello, greetings! I am recently working with MZmine 2 software for identification of plant metabolites. I have certain queries on setting the parameters to analyze my results. I tried using this...

21 September 2016 2,765 13 View