what difference will i observe in Gel-Doc say if i am using same sample for both the cases loading first lane-10 microlitre and 2 microlitre 6x loading dye and in 2nd lane case sample 2.5 microliter and dye- 0.5 microlitre , which case i a more and diluting why it will affect gel pattern, and how why more smears in first lane , why the second lane concentration is favoured why is it logical just because the dye is 6x