Hello,

If you have RNA contaminants even with the kit, it is essential to perform RNAse A decontamination, especially if the purity of your DNA sample is of prime importance for post-extraction manipulation.

This is to avoid any interference with RNA.

Yes, you must add RNase if you need pure DNA.

In order to get pure DNA with the DNAeasy Blood and Tissue Kit from Qiagen you need to add RNAseA during the purification steps before the elution.

Link:

https://www.protocols.io/view/manual-dna-extraction-using-qiagen-dneasy-blood-an-bi4dkgs6.pdf

@Roland kindich thank you for your suggestion.

I have been already adding RNaseA before elution.

But still I getting RNA contamination as bands are observed during gel visualization.

My only thought is can I add after DNA elution ,but the the logic fails here how would I remove the RNase from the eluted DNA

You can encrease the amount of RNAseA before elution or you can cut out one or multiple agarose gel DNA bands from the same sample and recover the DNA by pooling the gel bands with a DNA gel recovery kit and repeat the preparation step with DNAseA if required.

… and repeat the preparation step with RNaseA if required.

@Roland kindich ,Thank you for your kind suggestion