Sowmya Ramesh

11 Questions 7 Answers 0 Followers

Questions related from Sowmya Ramesh

What control should be used in Annexin apoptosis experiment?

While performing Annexin V and 7 AAD for identifying early and late apoptosis, what would be the ideal control? Is it recommended to add Annexin V and 7 AAD in the untreated samples and negate the...

16 June 2017 2,568 4 View

What could be the reason for observing a band in NTC in RTPCR?

We have been getting Ct values almost similar to a test sample in NTC wells. The melt curve also overlaps with the test samples. While running a gel, a band with a product size similar to the...

03 March 2017 5,245 3 View

Can I reuse the calcein labelled tissue within PMMA blocks for decalcification and perform routine histology?

I will be tracking the bone velocity in vivo using Calcein injection in animals. I understand that the sections should be fixed in 70% ethanol before embedding them in PMMA to get the sections....

15 March 2016 1,741 8 View

Can anyone suggest a protocol for RNA isolation from bones?

Usually I homogenize the bone fragments and proceed with conventional RNA isolation using TRI. But I find the purity of the RNA to be low. Which is the ideal method for storing bone samples and...

02 March 2015 4,962 7 View

What is the best way to transport RNA samples abroad?

Is there any other way apart from dry ice shipment that will maintain the integrity of the RNA samples?

18 October 2014 8,459 5 View

To what extent would the number of passage alter the miRNA expression?

There are many studies reporting the alteration in miRNA expression in the long-term cultures. But will there be any significant changes even when P2/P3 MSC cells are used for differentiation...

01 May 2014 7,560 1 View

Is there a possibility to screen the signaling molecules within bone marrow filtrate?

How to study the presence of a particular pathway in bone marrow filtrate?

31 March 2014 8,799 1 View

Is there any effective method to quantify DNA content within chitosan scaffolds?

In order to quantify DNA content, the chitosan scaffold is digested with papain. However, this results in less DNA content than the monolayer as the chitosan strongly binds the DNA. Is there any...

02 March 2014 1,011 1 View

Is there a possibility for the cell count to increase after cryopreservation?

Usually after cryopreservation the cells will have reduced viability. But for the first time, I found an increase cell number. I used 10% FBS, DMEM and DMSO for freezing. There was a 17% increase...

15 January 2014 7,110 3 View

What is the longevity of GFP expression after transplantation?

Is it possible to get the GFP labelled cells on histology 5 months after transplant?

31 December 2013 2,403 1 View

Where can we get collagen type 1 gel for human translation purposes?

I am looking for type 1 collagen that could be used for human translation or anything that is GMP manufactured/medical grade.

05 November 2013 6,633 2 View