Is there any effective method to quantify DNA content within chitosan scaffolds?

02 March 2014 1 1K Report

In order to quantify DNA content, the chitosan scaffold is digested with papain. However, this results in less DNA content than the monolayer as the chitosan strongly binds the DNA. Is there any other way to overcome this? Like pre-treatment using enzyme that releases the DNA from chitosan?

Ling Wang

chitosan strongly binds the DNA? you means that the chitosan can influence the fluoresence indensity? if it's ture, you need to change another methods to detect the DNA. if it's not, you can try tissue homogenization to pre-treat your scaffolds before digestion.

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