So-Jin Kim

12 Questions 17 Answers 0 Followers

Questions related from So-Jin Kim

How can I check the target promoter of my primers?

Hi all, I have primers for ChiP analysis in my lab. Before I start the experiments, I'd like to check this is really target for p16INK4A gene (CDKN2A). Q1. How can I check the target promoter...

04 May 2018 4,505 6 View

What is the reason for lines in separating gel?

Hi all ! I've used 30:1 acrylamide/bisacrylamide solution (Bio-Rad) for making WB gel. Once I run the gel, the line in separating gel suddenly showed up (please see the attached figure). It really...

11 January 2016 8,674 8 View

How could I distinguish real-signal from non-specific signal in immunofluorescence?

Hello. Recently, I perform immunofluorescence in liver tissue to detect co-localization of LC3 and LAMP, which are both autophagy-specific molecules. However, I can not find the differences...

25 July 2015 5,595 8 View

Is there commercial available cell-line for regulatory T lymphocyte?

I am investigating the effect of medicinal plants on regulatory T lymphocyte. To elucidate the molecular mechanisms, I plan to hire in vitro experiments. However, isolated regulatory T...

06 July 2015 3,957 0 View

What are the ethical methods for rabbit sacrifice?

Hello all ! I plan to sacrifice the rabbit for isolation of cartilage. In my laboratory, we usually used the anesthetic : Ketamine/xylazine and Ether. It is first experiment for me with...

07 April 2015 6,753 11 View

Specific inhibitor/inducer for mitochondria?

Hello all ! I am investigating the role of mitochondria and/or mitochondrial biogenesis in inflammatory diseases. I found the chemicals which protected against inflammatory diseases and induced...

06 April 2015 6,527 6 View

Are there tools to visualize necroptosis?

Hello Recently, I received the comments of referee that I should detect the TEM to confirm the necroptosis. However, I wonder there is a tool for this. Is there any histological and/or TEM...

04 April 2015 9,533 3 View

HPCL-grade water vs. nuclease free water vs. DEPC-treated water.

I plan to assay total cholesterol with "cholesterol assay kit". (Cayman, Cat.# 10007640) Instruments said to dilute the assay buffer 10X into 1X with HPLC-grade water. So, first I want to buy it,...

05 March 2013 5,744 28 View

Why do results show different expression patterns between mRNA and protein (ELISA)?

I'm interested in the relationship between HDAC (histone deacetylase) inhibitor (HDACi) and sepsis. With my recent experiment, I got different patterns between mRNA and ELISA as a result of some...

05 December 2012 731 3 View

Western blot band for Erk and phopho(p)-Erk

I want to know Erk activity in my animal model - sepsis. I isolated it from liver, homogenized it, separated it by SDS-PAGE, and detected it, but I just found one band for Erk. Procedure was the...

04 December 2012 7,071 17 View

Is there anyone who uses "Totallab" software for WB bands?

I used Totallab TL120 software for density analysis of WB bands. But sometimes I am faced with the problem of "inverted images". The software automatically converted band images to software...

03 December 2012 5,476 3 View

Primer for mouse MKP-1 DNA

It is the first time I order primer for specific DNA. I want to make a primer (reverse PCR) for mouse MKP-1 DNA and I don't know how can I make it! Can I have any information needed to make...

11 May 2012 2,494 4 View