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Hi, I'm relatively new to this coupling reactions so studying details of the reactions. I just wonder why we need to cool down the mixture of DMAP/carboxylic acid/alcohol or amines to zero...
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Hi, I am conducting experiments with mammalian cells, for now 3T3s. I wanted to wash the cells after coating in polyelectrolyte aqueous solutions, and, as I have always done, I centrifuged down...
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I work on polymer gels and composites. When I did frequency sweep on my samples (particles in hydrogels), interestingly, the G' and G" increased with frequency after certain frequency. So, the...
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Hi I'm trying to stain the inflammatory cells or immune cells (both or either is okay) from in vivo tissue sections. I implanted the polymeric scaffolds (polycaprolactone, PCL) into rats. From...
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I've stained the dead and live cells with the live/dead cell assay from Life Technologies, but the kit is not suitable for further fixation according to the manufacturer. I seeded the cells on the...
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