I am performing in-gel digestion. I wanted to know exact chemistry of the process by which 100% acetonitrile dries up the gel and turn it yellow.
The acetonitrile dissolves the water out of the gel which is a polymer that is not soluble in acetonitrile, and this causes the observed dehydration.
Extract acetonitrile, butyl acetate. Then dry under vacuum.
https://www.researchgate.net/publication/257561673_Recovery_of_acetonitrile_from_aqueous_waste_by_a_combined_process_Solvent_extraction_and_batch_distillat
Best regards
Article Recovery of acetonitrile from aqueous waste by a combined pr...
@Yuri Mirgorod I dont think thats what i m looking for..
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