Dear All, I am want to screen my compounds for rescue against Glutamate induced excocitotoxicity on rat primary cortical neurons. I referred few papers suggesting glutamate exposure for 24 hrs (at concentrations as low as 8 micromolar), however, few papers suggest acute exposure like 30 min. or 50 min. with high concentration of Glutamate such as 100 micromolar. Can some one suggest me the suitable concentration as well as exposure time which may help in screening my molecules?

I have attached 2 papers reporting the same as described in question

Regards

Shubham

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