Can anybody help me with a protocol for Sudan Black B staining for paraffin sections?

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Juan Manuel Herrera

Hello Shaima,

I never did Sudan Black staining, but I can recommend this web to see a protocol for Lipofuscin https://webpath.med.utah.edu/HISTHTML/MANUALS/MANUALS.html

Maybe you could try it.

Also, I recommend you to look for Bancroft's Theory and Practice of Histological Techniques book; it's very complete.

Good luck,

Juan