Shadid Uz Zaman

12 Questions 11 Answers 0 Followers

Questions related from Shadid Uz Zaman

Is there any formulas or equations to calculate the dose of a drug for transdermal and topical patch preparation?

I want to prepare a topical and a transdermal patch system of a certain drug which is only available orally at the moment. How can I calculate the dose of the drug for the patch system? For...

05 May 2019 8,510 7 View

Can anybody suggest me literature to prepare 6-hydroxynaphthalene-2-sulfonic acid from 2-naphthol?

I want to design a simple synthesis method that can be carried out by undergrad students at a synthesis lab. I can't find definitive literature about the procedure in rigorous details. All that I...

09 September 2018 5,401 1 View

It is possible to synthesize Procaine from TEA and PABA?

I am trying to develop a simple Organic Chemistry Lab that will teach students on how to conduct Esterification reaction. The catch is that the synthesized molecule should have recognized...

07 July 2018 7,505 2 View

How can I obtain the article by Bauer and Kirby regarding their method of disc diffusion assay?

I am trying to download the original paper by Bauer and Kirby, 1966 regarding their method. But with browsing the journal, it shows articles up to page 492, but the article is at page 493-496. I...

01 January 2018 302 0 View

Is it possible to develop a rudimentary method of estimating gene expression using Normal PCR?

I have no expertise with PCR at all, but I do understand the principle behind PCR, qPCR and RT-PCR. I want to estimate the expression of certain gene in bacteria. But right now, I only need a...

12 December 2017 3,541 6 View

How reliable is Toxicity Estimation Software Tool?

I have come across a software called Toxicity Estimation Software Tool (TEST) in the EPA website. They predict mutagenicity, developmental Toxicity etc. I would like to know if anyone here used it...

11 November 2017 1,703 4 View

Is there any primer that can be used for molecular identification of E. coli, Staph. aureus, and S. typhi?

I want to confirm the identity of some clinical isolates of E. coli, Staph. aureus, and S. typhi using PCR. I am looking for a single pair of primers which can be used to produce PCR product of...

11 November 2017 8,275 4 View

How to select solvent for stock antibiotic solution preparation?

I am trying to determine MICs for several clinical isolates for Linezolid, Amikacin sulfate, Ciprofloxaxin HCl, Cefixime trihydrate, and Chloramphenicol. But I am having problem preparing the...

11 November 2017 8,560 2 View

How to identify E. coli conclusively?

I currently have 16 multidrug resistant strains of E. coli (supposedly). But I need to ensure they ARE actually E. coli. I have been directed to IMViC test which doesn't seem conclusive or...

09 September 2017 2,089 8 View

IsPreparation of Tryptic Soy Agar from TSB and Agar possible?

I have purchased commercial pack of Tryptic Soy Broth. I already have Agar Powder available in my Lab. Now I need TSA for preserving bacteria using Agar slant/Mineral oil method. I don't want to...

09 September 2017 1,640 3 View

Is preparation of chromic acid from potassium/sodium dichromate possible?

I want to use chromic acid as an oxidizing agent in an organic reaction, but I don't have chromic oxide. I have potassium dichromate and sodium dichromate in large amount though. So, I started...

09 September 2017 2,725 3 View

Is it possible to fractionate small peptides (<50AA) using analytical HPLC?

I am currently developing a research protocol involving antimicrobial peptides. I have searched various journal articles, and they describe that to purify these peptides I need to have sep-pak...

01 January 2017 8,252 3 View