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Questions related from Sergio Fandiño González
So I have noticed for a while that for the same plasmid, sometimes it can be seen in a gel in only one conformation, often two and sometimes three or more. Sometimes samples of the same plasmid...
20 May 2024 5,839 6 View
Hello, I need to use a program that only takes gbk as input. I am working with viral sequences that are not under "genomes" but under "nucleotide" category in genbank. I need to compare those with...
25 October 2023 5,594 1 View
Hello, I am working with closely related endogenous retrovirus (ERV) sequences. I suspect these might have been the result of several integration events from different but related exogenous...
26 September 2023 7,007 3 View
Hello, we are doing essays inducing promoters with IFN alpha A/D and alpha-2a. Sometimes we get results saying that a certain promoter is induced from 1600U/ml and upwards in mammals for...
29 May 2023 6,278 0 View
Hello, I have recently read about the reverse transcription activity of one of the subunits of telomerase. As far as I know, all reverse transcriptases so far are of viral origin, including the...
14 April 2023 5,822 2 View
Nearly all recent bibliography I consulted remains constant about ALV-A, B, C, D, J and K viruses being exogenous infective and ALV-E being an endogenous virus that is transmitted through genetic...
27 October 2021 8,073 0 View
While reading an old paper (https://core.ac.uk/download/pdf/82090013.pdf , 1995) about a similar virus to the one Im working with, I read they found a binding site for VBP...
11 October 2021 5,548 0 View
Hello, I have designed a pair of primers to amplify a part of an endogenous retrovirus that is present in all individuals of the target species with each individual having several copies. Im...
15 June 2021 2,338 2 View
While there are a lot of Avian Leukosis Virus (ALV) sequences in databases, there are fewer from Rous Sarcoma viruses (RSV). ALV is grouped within subgroups A to K except F, G, H and I. RSV is a...
24 May 2021 9,149 5 View
I'm interested in fish genetics and phylogenogenetics. I might choose this topic for my PhD, however I have a concern of how samples are acquired for these kinds of studies. For fishes >8-10...
10 October 2019 426 3 View
I'm a student doing a experiment and I need 2 strains, one F+ and one F-. In my institution they only have antibiotic-resistant strains not suited for this type of experiment, so I have to buy...
20 July 2018 7,663 2 View
I got some M13 phage in my undergraduate practices and I was thinking about trying to isolate E. coli from human intestine to culture it at home with a homemade culture medium. But for that I need...
27 May 2018 3,609 2 View
Hello, My topic of research is Avian Leukosis Virus (ALV). I have noticed that there are some old research papers that showed that when injecting chicken cells infected with Rous Sarcoma Virus (a...
01 January 1970 1,256 1 View
