Sergio Fandiño González

13 Questions 4 Answers 0 Followers

Questions related from Sergio Fandiño González

What environment factors affect plasmid conformation in a lab?

So I have noticed for a while that for the same plasmid, sometimes it can be seen in a gel in only one conformation, often two and sometimes three or more. Sometimes samples of the same plasmid...

20 May 2024 5,837 6 View

How to download nucleotide sequences as a gbk file?

Hello, I need to use a program that only takes gbk as input. I am working with viral sequences that are not under "genomes" but under "nucleotide" category in genbank. I need to compare those with...

25 October 2023 5,592 1 View

Is it possible to do an statistical test to compare single integration versus multiple integrations of related endogenous retroviruses?

Hello, I am working with closely related endogenous retrovirus (ERV) sequences. I suspect these might have been the result of several integration events from different but related exogenous...

26 September 2023 7,002 3 View

What is the top limit of IFN alpha concentration in units (U) per volume in mammals and avians?

Hello, we are doing essays inducing promoters with IFN alpha A/D and alpha-2a. Sometimes we get results saying that a certain promoter is induced from 1600U/ml and upwards in mammals for...

29 May 2023 6,276 0 View

Eukariotic TERT (Telomerase reverse transcriptase) related to viral reverse transcriptases?

Hello, I have recently read about the reverse transcription activity of one of the subunits of telomerase. As far as I know, all reverse transcriptases so far are of viral origin, including the...

14 April 2023 5,821 2 View

In old papers, ALV-E is referred to as an infective exogenous virus, why?

Nearly all recent bibliography I consulted remains constant about ALV-A, B, C, D, J and K viruses being exogenous infective and ALV-E being an endogenous virus that is transmitted through genetic...

27 October 2021 8,072 0 View

Can transcription factor binding sites work in the complementary strand? Do known TBS sequences change drastically with new research?

While reading an old paper (https://core.ac.uk/download/pdf/82090013.pdf , 1995) about a similar virus to the one Im working with, I read they found a binding site for VBP...

11 October 2021 5,547 0 View

PCR with same samples, in the same conditions, sometimes amplify others don't?

Hello, I have designed a pair of primers to amplify a part of an endogenous retrovirus that is present in all individuals of the target species with each individual having several copies. Im...

15 June 2021 2,337 2 View

What exactly is the relationship between ALV and RSV viruses?

While there are a lot of Avian Leukosis Virus (ALV) sequences in databases, there are fewer from Rous Sarcoma viruses (RSV). ALV is grouped within subgroups A to K except F, G, H and I. RSV is a...

24 May 2021 9,149 5 View

Fish DNA extraction=death?

I'm interested in fish genetics and phylogenogenetics. I might choose this topic for my PhD, however I have a concern of how samples are acquired for these kinds of studies. For fishes >8-10...

10 October 2019 425 3 View

Where can I buy Escherichia coli strains for cheap?

I'm a student doing a experiment and I need 2 strains, one F+ and one F-. In my institution they only have antibiotic-resistant strains not suited for this type of experiment, so I have to buy...

20 July 2018 7,661 2 View

Does anyone know if M13 Phage resistance is widespread in human E. coli?

I got some M13 phage in my undergraduate practices and I was thinking about trying to isolate E. coli from human intestine to culture it at home with a homemade culture medium. But for that I need...

27 May 2018 3,607 2 View

Tumors caused by oncoviruses of different species lacking cell receptors?

Hello, My topic of research is Avian Leukosis Virus (ALV). I have noticed that there are some old research papers that showed that when injecting chicken cells infected with Rous Sarcoma Virus (a...

01 January 1970 1,254 1 View