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Questions related from Sepideh Parsi
Hi imaging gurus! I am looking for a simple method to get statistics on two or three signals colocalizations on a Z-stack file. I have used imageJ but sometimes it gives me an error and other...
10 October 2019 8,767 2 View
I am facing with a perplexing situation in which there are two proteins both identifying correct protein sizes in immunoblot by polyclonal antibody (monoclonal doesn't exist in market) but it is...
07 July 2019 6,029 4 View
Is it recommended to extract RNA from the same cells treated with XTT after seeing the viability results or are people supposed to run a separate experiment for studying RNA? I thought maybe it...
01 January 2015 9,460 5 View
I noticed there are many articles working on this subject, but the method specifically in preparation of Abeta is slightly different from article to article. There were discrepancies about using...
09 September 2014 9,003 4 View
I have used Antibody sc-133607 as an example here . According to the company sample of WB in cell line it gives two bands bigger than 90kD and 144kD the latter I suppose is the predicted size of...
07 July 2014 8,214 4 View
After 48hrs I do pr extraction from n2a cells which had been transfected with lipofectamine+50uM scramble RNA ctrl previously. The phenomena has been repeated so that I see a bad reduction in my...
01 January 2014 8,296 1 View
The formula for this calculation is Efficiency = -1+10(-1/slope),Slopes between -3.1 and -3.6 giving reaction efficiencies between 90 and 110% are typically acceptable. I calculated my slope -2.5...
11 November 2013 5,137 1 View
