Seong jun Lee

5 Questions 3 Answers 0 Followers

Questions related from Seong jun Lee

Have anyone ever tried cell culture with Y23967 (Rho kinase inhibitor) added?

It is described in the literature that Y27632 proliferates cells and inhibits differentiation. Cell adhesion is also good. But when we experimented, there was no clear difference. I was...

06 June 2017 1,576 3 View

Keratinocyte culture treatment with a low concentration of Cholera toxin or mitomycin C causes keratinocyte damage?

When keratinocytes lacking maternal cells are cultured, does keratinocyte damage occur by treating mitomycin C(4ug/ml) and cholera toxin(10^-10M)? Or inhibit the growth of keratinocyte?

03 March 2017 6,251 1 View

How to remove fibroblast?

Keratinocy grows together with fibroblast. I am using keratinocyte growth media. Is there a way to remove fibroblast?

03 March 2017 2,629 5 View

Help me prevented contamination with HaCaT cells?

We was received some labolatories HaCaT cell. And Immediately, I went mycoplasma test performed. All were positive reaction(contamination). But Other kind of cells were not contaminated. If anyone...

03 March 2017 6,640 2 View

Can keratinocyte shape change after keratinocyte subculture?

We are cultivating keratinocyte. However, when the subculture is carried out, the shape of the keratinocyte becomes elongated. Keratinocyte was cultured in the same manner as before the...

01 January 1970 9,288 6 View