Sean M Hoban

9 Questions 29 Answers 0 Followers

Questions related from Sean M Hoban

Good machine for plant cell lysis and DNA extraction? Quiagen/ Fastprep/ Genogrinder?

Can anyone compare/ contrast the Fastprep vs. Tissue Lyser grinders? https://www.qiagen.com/us/shop/automated-solutions/sample-disruption/tissuelyser-ii/ … and...

10 October 2016 9,229 0 View

What is best sealant for PCR plates?

Hey lab folks, for PCR do you prefer disposable (plastic or metal, one time use like https://www.fishersci.com/shop/products/corning-sealing-mat-384-well-polypropylene-storage-plates/07202500) or...

09 September 2016 3,965 9 View

#Botanist friends: anyone ever had problems transporting leaves, seeds or soil while flying within continental US? What about shipping USPS?

Colleagues: has anyone ever had problems transporting leaves, seeds or soil while flying within continental US (from state to state, never leaving the country)? What about shipping USPS? I'm...

07 July 2016 6,581 0 View

What DNA extraction protocol must be used for RADSeq next generation DNA sequencing (NGS), for plant tissue?

I am planning a potential RADSeq project (my first one). I've heard from some people that RADSeq requires quality DNA samples (very high molecular weight, very clean, high concentration)-...

03 March 2016 3,709 12 View

Which tissue homogenizer to use for plant cell disruption and DNA extraction (fastprep, miniG, beadbeater, retsch, precylls.. so many!)?

I am planning to purchase a cell disruptor/ homogenizer, probably the kind that shakes beads (steel, glass, or ceramic) at very high speeds, to break open plant leaf (and possibly wood or seeds)...

03 March 2016 5,616 14 View

What do you think about 'Peerage of Science'?

Just curious what other scientists think about the "fully open" and "peer review of peer review" website Peerage of Science. If you don't know about it, here are a few links explaining...

12 December 2013 8,044 3 View

What are some resources for postdocs who are currently funded for salary but would like to seek additional funding?

I would like to compile a list of grant opportunities that will be useful for myself and for other postdocs in ecology and evolutionary biology (non bio-medicine). I am specifically interested in...

08 August 2013 1,550 10 View

What population genetic software should I to use to calculate allelic richness?

To my knowledge, the two most popular software packages for analyzing population genetic data (Arlequin and Genepop) do not calculate allelic richness, which is corrected for sample size. Fstat...

08 August 2013 3,030 10 View

What is a valid statistical test for comparing samples taken from a population over time?

I would like to take temporal samples of DNA from a population over time to determine whether a decline in various measures of genetic diversity is occurring. For example, samples taken at time...

06 June 2013 7,196 10 View