I tried to set up RPA with gp32, bst taq polymers and uvsX but without uvsY, Ck and PC. But I don't have any band in gel electrophoresis. I cleaned up RPA amplification by PCR clean-up kit. But there is no band. I need to know uvsY, Ck and PC are optional or no?
And how much is concentration of gp32 and uvsX in recombinase polymerase amplification reaction?