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Questions related from Reid Oldenburg
We have conducted a prospective trial. A lesion was split into 2 parts. Part (A) was injected with treatment and Part (B) was injected with control/vehicle. Part (A) and (B) were tracked at 4 time...
05 September 2023 5,957 2 View
Does anyone know what media to put keratinocytes in with PBMCs? what if I co-culture KCs and PBMCs together for 24 hours? I am interested in looking at keratinocyte mortality but am worried that...
07 May 2021 235 0 View
I would like to freeze a 4mm skin biopsy. After thawing, will these keratinocytes have working MHCI that can interact with TCR on CD8+ cytotoxic T cells?
31 July 2020 8,754 0 View
Our lab has previously shown that iNOS expression does not occur in TRIF KO murine BMDMs stimulated with LPS-IFNg. A previous publication showed that LPS does not stimulate nitric oxide...
19 December 2017 3,624 0 View
I'd like to quantify total TRIF protein in my murine macrophages. My previous experiments using an anti-TRIF polyclonal ab in Western blotting have given me unclear data because I have multiple...
19 February 2016 9,315 1 View
I have seen reports of TLR2 or TLR4 directly interacting with NOX subunits to cause the production of ROS. If anyone has a couple of good articles that speak of this interaction, or of other...
20 November 2014 3,443 6 View
I am using CD3/28 Dynabeads to activate mouse primary T cells in vitro, which causes aggregation of my cells. When I do flow cytometry, I have large aggregates of cells, which I cannot use for...
04 November 2014 10,024 5 View
