Reid Oldenburg

10 Questions 7 Answers 0 Followers

Questions related from Reid Oldenburg

What statistical method should I use to track treatment vs control at multiple timepoints?

We have conducted a prospective trial. A lesion was split into 2 parts. Part (A) was injected with treatment and Part (B) was injected with control/vehicle. Part (A) and (B) were tracked at 4 time...

05 September 2023 5,964 2 View

PBMC and keratinocyte co-culture conditions?

Does anyone know what media to put keratinocytes in with PBMCs? what if I co-culture KCs and PBMCs together for 24 hours? I am interested in looking at keratinocyte mortality but am worried that...

07 May 2021 240 0 View

Do frozen keratinocytes maintain functional surface MHCI expression?

I would like to freeze a 4mm skin biopsy. After thawing, will these keratinocytes have working MHCI that can interact with TCR on CD8+ cytotoxic T cells?

31 July 2020 8,759 0 View

INOS production in TRIF (Ticam1) KO macrophages stimulated with LPS-IFNg?

Our lab has previously shown that iNOS expression does not occur in TRIF KO murine BMDMs stimulated with LPS-IFNg. A previous publication showed that LPS does not stimulate nitric oxide...

19 December 2017 3,627 0 View

Do mouse Schwann cells produce interferon-Beta?

I have noticed that Schwann cells make MCP-1, IL-1beta, IL-6 and TNF upon LPS stimulation. I also know that SCs express TLR4, so I have been successfully stimulating with LPS. Do SCs produce...

02 August 2016 4,822 0 View

Anyone have a good way to quantify total levels of TRIF (Ticam1) using Western blotting or FACS (in murine macrophages)?

I'd like to quantify total TRIF protein in my murine macrophages. My previous experiments using an anti-TRIF polyclonal ab in Western blotting have given me unclear data because I have multiple...

19 February 2016 9,319 1 View

Is there an antibody for intracellular MyD88 total protein detection using flow cytometry?

I have already done western blotting, but would like to detect total MyD88 levels in BMDMs using flow cytometry. I am planning on using a standard protocol to permeabilize my macrophages before...

02 October 2015 3,393 1 View

How do TLRs stimulate NADPH oxidase activity?

I have seen reports of TLR2 or TLR4 directly interacting with NOX subunits to cause the production of ROS. If anyone has a couple of good articles that speak of this interaction, or of other...

20 November 2014 3,448 6 View

Do Dynabeads compromise FACS analysis?

I am using CD3/28 Dynabeads to activate mouse primary T cells in vitro, which causes aggregation of my cells. When I do flow cytometry, I have large aggregates of cells, which I cannot use for...

04 November 2014 10,041 5 View

Anyone know of a good assay to measure antioxidant/free radical scavenging properties of a molecule?

I am working with a phenolic compound that may act as a free-radical scavenger within macrophages, reducing the initiation of apoptosis. I have seen reports that test the free radical scavenging...

18 September 2014 3,120 16 View