Reid Oldenburg

5 Questions 4 Answers 0 Followers

Questions related from Reid Oldenburg

What statistical method should I use to track treatment vs control at multiple timepoints?

We have conducted a prospective trial. A lesion was split into 2 parts. Part (A) was injected with treatment and Part (B) was injected with control/vehicle. Part (A) and (B) were tracked at 4 time...

05 September 2023 5,949 2 View

Do frozen keratinocytes maintain functional surface MHCI expression?

I would like to freeze a 4mm skin biopsy. After thawing, will these keratinocytes have working MHCI that can interact with TCR on CD8+ cytotoxic T cells?

31 July 2020 8,751 0 View

INOS production in TRIF (Ticam1) KO macrophages stimulated with LPS-IFNg?

Our lab has previously shown that iNOS expression does not occur in TRIF KO murine BMDMs stimulated with LPS-IFNg. A previous publication showed that LPS does not stimulate nitric oxide...

19 December 2017 3,622 0 View

Anyone have a good way to quantify total levels of TRIF (Ticam1) using Western blotting or FACS (in murine macrophages)?

I'd like to quantify total TRIF protein in my murine macrophages. My previous experiments using an anti-TRIF polyclonal ab in Western blotting have given me unclear data because I have multiple...

19 February 2016 9,313 1 View

Do Dynabeads compromise FACS analysis?

I am using CD3/28 Dynabeads to activate mouse primary T cells in vitro, which causes aggregation of my cells. When I do flow cytometry, I have large aggregates of cells, which I cannot use for...

04 November 2014 10,022 5 View