14 Questions 24 Answers 0 Followers
Questions related from Ravi Kanth Reddy Sathi
I am calculating the correlation values between two data sets of size 257. I want to know what is the critical value of correlation for a sample size of 257. I tried searching on the web, but...
12 December 2013 9,205 18 View
I am trying to find out existence of protein domains in a set of sequences. I am using BLASTX for the task. I have made a BLASTX of my sequences with the ProDom sequences. I used an e-value cutoff...
11 November 2013 4,673 3 View
ITS regions are used for identifications of bacterial species. But while observing bacterial genomes it is seen that tRNA sequences are present in the ITS regions present between 16S rRNA and 23S...
10 October 2013 4,096 5 View
I have a set of aligned sequences in fasta format. I want to get consensus out of the alignment. In case of most of the sites one of the base is showing maximum occurrence. In case of sites where...
10 October 2013 3,861 2 View
I am working with around 2600+ genomes and wish to study the genome, gene and intergenic features among various groups. In case of taxonomical groups which have very few representatives, there is...
09 September 2013 9,566 6 View
I am working on finding the functional signatures/remains of regulatory regions, RNAs and proteins in the intergenic sequences (IGs) of E. coli K12 MG1655. I have a few doubts regarding the...
08 August 2013 1,863 1 View
I work on E. coli genomes and while going through the various genes present, I have seen (link) that in the coordinates area of the description it is suggested to join different regions of genome....
08 August 2013 9,141 7 View
I have tried running a command line blast. The query file is a multi fasta file containing 2600 sequences. It was made a BLASTX against a proteins sequences (ProDom) of size 2 GB (prodom.phr :...
08 August 2013 423 0 View
I am working at genomes level and I have a shortage of computational resources to do the tasks I want to. So could you please suggest where I can get free online access to a good system for my...
08 August 2013 9,250 5 View
Out of my experience I think the basic difference among both of them lies in speed and usage. PHP can be used for creating online tools. PERL can also be used, but PHP is more easy to handle to...
07 July 2013 500 4 View
Can some one please throw some light on cloud facilities available to carry on Bioinformatics work. Also is there a possibility of using such services for free or for a nominal fee, for academic...
07 July 2012 5,059 19 View
Whether it be an automated genome annotation or an analysis which involves huge data, we need to know the computational resources needed for the task. Can anyone please suggest how to estimate...
07 July 2012 9,707 10 View
Could some one suggest some good standalone tools for protein structure prediction which are reliable. I want to use them in combination with a few other tools for automated annotation.
02 February 2012 1,932 7 View
I am trying to compile a code for running Needleman Wunsch algorithm using PHP. I could come up to matrix construction and filling. I don't understand how to write the logic for back tracking so...
02 February 2012 4,362 11 View
