9 Questions 3 Answers 0 Followers
Questions related from Rajesh Kumar
Dear ResearchGate community, I like to attach Indole-3-acetic acid (3-IAA) to the N-terminus of my peptide. Can I directly couple it using my Microwave Peptide Synthesizer (Liberty Blue, CEM), or...
07 November 2023 435 0 View
Dear ResearchGate Community, I am currently facing a challenge in the LC-MS analysis of a highly hydrophobic peptide (already purified to >98% by a manufacturer) on our 1260 LC-MS System. The...
15 October 2023 736 6 View
I like to do SPPS by Fmoc/tBu chemistry to make a peptide (Z-Leu-Lys-Glu-Ala-OH) with an N-terminal-CBZ/Z group at Leu. I like to use Z- Leu-OH for the last coupling, but after the synthesis, I...
12 June 2023 324 1 View
Dear Colleagues, How can I synthesize a peptide with N-terminal protection by the benzyloxycarbonyl group (Z-group) using Fmoc/tBu Solid-Phase Peptide Synthesis? Can Z-Leu-OH be coupled during...
09 June 2023 3,454 1 View
Dear colleagues, Can we synthesize Alpha-Defensin-4 (Human; 33-mer) peptide with 3 disulfide bonds between Cys2-Cys30, Cys4-Cys19 and Cys9-Cys29 using the Microwave Liberty Blue peptide...
28 April 2023 588 0 View
Dear colleagues, I´ve one question regarding MS noise spectra in LC-MS. Actually, I am getting spectral (background noise?) peaks in both blank and water (-ve control) HPLC runs on Agilent´s...
05 January 2023 8,539 0 View
Dear researchgate community, Is there any good method/idea to prevent aspartimide formation during Microwave-assisted peptide synthesis (e.g., on CEM Liberty Blue)? My peptide (28-mer) has...
18 December 2022 7,527 3 View
Does anybody know a good antibody for yeast mt-DNA encoded proteins? I could not find a company that makes such an antibody (e.g. COX1, COX2 and COX3). Thanks
17 March 2019 4,587 2 View
Dear colleagues, I would like to do the BOC (tert-butyloxycarbonyl) protection on the indole ring of "Indole-3-acetic acid (3-IAA)" to get "N-Boc indole-3-acetic acid". Does anyone have an idea...
01 January 1970 2,161 0 View
