How to store rodent platelets?

Propanna Bandyopadhyay @Propanna-Bandyopadhyay

22 September 2020 0 2K Report

Currently, I am isolating the platelets from Wistar rats (female) by retro-orbital puncture & using ACD buffer as an anticoagulant. Can someone please suggest me the storage conditions that should be maintained. I have tried storing them in incubators having conditions 25 degrees C, 5% CO2 & 21% O2 but this is leading to change in the morphology of platelets & also platelet aggregation.

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