Hi, Can anyone suggest me the extraction protocols from saliva .I don't want to use the kit method since it is costly.Please suggest me?

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P-nitrophenol acetate assay for esterase and media supernant colour interference. what can be done?

I am performing p nitrophenol acetate assay for esterase enzyme. My media contains a different concentration of peptone (0.5, 2, 5%) and after centrifugation the supernatant is slight yellow....

16 June 2024 4,381 0 View

Is there any open access software like sci finder to know about published paper?

Sci finder helps to find out the research that has published.

30 April 2024 6,287 0 View

Can any one tell me the indirect method to calculate SOD based on Quercetin?

Full method and formula to calculate SOD based on Quercetin in animal tissue

08 March 2024 6,303 0 View

Wireless smart car using embedded system and iot related research or journals?

related to microcontroller and sensors

12 January 2024 692 2 View

Can anyone guide how to use the minitab software for optimization studies ?

I want to learn minitab software and how to use that software for optimization studies of the enzyme. How we can find the actual and predicted values from the software.? how we can make Plackett...

23 September 2023 5,983 2 View

Which statistics should I prefer, if normality and homogeneity principal has met but the two groups have unequal sample sizes of 12 and 58?

please answer.

18 August 2023 1,996 7 View

What could be the reason for NO separation of Biodiesel and Glycerol from fresh vegetable oil tried by using CaO (LR grade) as well as KOH?

I'm stuck at a point where previously I used to get a clear separation of the Biodiesel and glycerol from used cooking oil (checked FFA + esterified) using CaO nanoparticles as the catalyst, but...

12 August 2023 1,523 1 View

Can anyone help me to identify this marine tanaidaceans?

The crustacean order Tanaidacea consists of macrobenthic invertebrates, which constitute a major food source for higher trophic levels in estuarine and marine environment.

27 July 2023 385 2 View

Can anyone identify Gnathia species isopod?

Gnathia is a genus of marine isopod crustaceans.

31 May 2023 9,906 2 View

Why BSA protein is used for fouling analysis of polymer membrane?

Is there any other material than BSA that could be used for the fouling analysis of polymer membrane when it is used for the emulsion separation experiment? What is the reason behind selecting...

17 April 2023 6,970 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View