Prabhuraj R S

15 Questions 25 Answers 0 Followers

Questions related from Prabhuraj R S

What is the advantage of intraperitoneal injection over intravenous injection in mice?

I am working on nanoparticles for the treatment of cancer. We are planning to do antitumor efficacy in mice. People generally use intravenous route to inject nanoparticles to mice. I would like to...

01 April 2019 4,255 3 View

Why calculation of drug loading % of HPLC is not matched with UV?

I am synthesizing the plga nanoparticles with an anticancer drug. I took 100 microlitre of drug loaded plga nanoparticles and add 900 microlitre of acetone to it. Then I sonicate the drug loaded...

27 December 2017 1,300 2 View

How to peel plga film from the glass or plastic plate?

I am trying to plga scaffold for drug delivery application. I dissolved 500 mg plga in 50 ml acetone. Then I poured 10 ml of plga solution to glass petridish and 10 ml of plga solution to plastic...

27 December 2017 9,188 3 View

How to avoid the loss of dead cells in cell cycle analysis using flow cytometry?

I am doing cell cycle analysis using Propidium iodide dye in flow cytometry. My samples are 1) cells 2)cells+ silica 3) cells+ drug 4) Cells+ silica-drug The cells treated for 24 hrs with the...

14 December 2017 4,521 2 View

How to leach out the dual drug loaded chitosan coated Plga system?

First I synthesise the Plga nanoparticles with loading of Hydrophobic drug in it. Then I use chitosan to coat the hydrophobic drug loaded plga nanoparticles. while the coating the chitosan over...

10 November 2017 8,224 0 View

How to find amount of graphene attached with mesoporous silica nanoparticles?

I synthesised the mesoporous silica nanoparticles. Then I attached graphene nanoparticles with mesoporous silica nanoparticles. TEM images clearly show the attachment of graphene over mesoporous...

02 November 2017 1,444 21 View

How much amount of glutaraldehyde can be used to crosslink amine functionalized mesoporous silica with chitosan?

I am using 20 mg of mesoporous silica with 500 micro litre of glutaraldehyde and 20 mg of chitosan dispersed in 2% of glacial acetic acid. MTT assay showed that after 100 microgram of chitosan...

02 May 2017 342 4 View

Which solvent is best to load the hydrophobic drug into mesoporous siica?

The drug is hydrophobic . The solubility of drug is less than 0.5 mg/ml in water, methanol and ethanol. But drug has very good solubility in DMSO. Is it good to use DMSO as the only solvent to...

31 July 2016 2,638 5 View

Thermogravimetric analysis (TGA) can be used to find the amount of drug loaded in nanoparticles?

I am working on mesoporous silica nanoparticles. One can find the loading and entrapment efficiency using UV method. For example, I did the TGA for mesoporous silica from 30-900 degree celsius and...

28 July 2016 9,357 9 View

Whether amount of loading time have an effect of drug loading in nanoparticles?

I am working on nanoparticles for the treatment of cancer. I am working with hydrophobic and hydrophilic drug. usually people (in papers) use 24 hrs for loading of drug in nanoparticle. My...

24 July 2016 1,764 7 View

How to understand percentage of cancer cell growth inhibition and percentage of cancer cell killing in the same graph?

The graph show cyctoxicity of different combination of chemo drugs on a cancer cell line. The graph compare the percentage of cancer cell growth inhibition and percentage of cancer cell killing...

12 July 2016 8,647 3 View

How to find number of cancer cells are there?

I plotted standard cancer cell growth curve for 24, 48, 72, 96 hrs. The cell concentration varies from 5000 to 50000 cells. For example, initially I seeded 10000 cells to 96 well plate. After...

11 July 2016 159 10 View

Gemcitabine HCl is not loading into mesoporous silica nanoparitciles?

I am loading Gemcitabine HCl into mesoporous silica nanopariticles. I took 25 mg of mesoporous silica nanoparticles and 10 mg of drug for loading purpose. I used PBS 7.4 as a medium to load the...

01 January 1970 9,156 7 View

Whether PH play an important role in loading of drug into the nanoparticles?

If we change the PH of the solution from 7.4 to 9, it will affect the drug loading percentage in nanoparticles.

01 January 1970 9,581 3 View

How to coat polyethylene glycol over the surface of the mesoporous silica nanoparticles?

I am working on mesoporous silica nanoparticles for the treatment of pancreatic cancer. I am planning to put PEG over the surface of the mesoporous silica nanoparticles. I want good suggestions...

01 January 1970 2,140 3 View