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Questions related from Prabhuraj R S
I am working on nanoparticles for the treatment of cancer. We are planning to do antitumor efficacy in mice. People generally use intravenous route to inject nanoparticles to mice. I would like to...
01 April 2019 4,249 3 View
I am synthesizing the plga nanoparticles with an anticancer drug. I took 100 microlitre of drug loaded plga nanoparticles and add 900 microlitre of acetone to it. Then I sonicate the drug loaded...
27 December 2017 1,294 2 View
I am trying to plga scaffold for drug delivery application. I dissolved 500 mg plga in 50 ml acetone. Then I poured 10 ml of plga solution to glass petridish and 10 ml of plga solution to plastic...
27 December 2017 9,182 3 View
I am doing cell cycle analysis using Propidium iodide dye in flow cytometry. My samples are 1) cells 2)cells+ silica 3) cells+ drug 4) Cells+ silica-drug The cells treated for 24 hrs with the...
14 December 2017 4,516 2 View
First I synthesise the Plga nanoparticles with loading of Hydrophobic drug in it. Then I use chitosan to coat the hydrophobic drug loaded plga nanoparticles. while the coating the chitosan over...
10 November 2017 8,219 0 View
I synthesised the mesoporous silica nanoparticles. Then I attached graphene nanoparticles with mesoporous silica nanoparticles. TEM images clearly show the attachment of graphene over mesoporous...
02 November 2017 1,436 21 View
I am using 20 mg of mesoporous silica with 500 micro litre of glutaraldehyde and 20 mg of chitosan dispersed in 2% of glacial acetic acid. MTT assay showed that after 100 microgram of chitosan...
02 May 2017 334 4 View
The drug is hydrophobic . The solubility of drug is less than 0.5 mg/ml in water, methanol and ethanol. But drug has very good solubility in DMSO. Is it good to use DMSO as the only solvent to...
31 July 2016 2,630 5 View
I am working on mesoporous silica nanoparticles. One can find the loading and entrapment efficiency using UV method. For example, I did the TGA for mesoporous silica from 30-900 degree celsius and...
28 July 2016 9,352 9 View
I am working on nanoparticles for the treatment of cancer. I am working with hydrophobic and hydrophilic drug. usually people (in papers) use 24 hrs for loading of drug in nanoparticle. My...
24 July 2016 1,749 7 View
The graph show cyctoxicity of different combination of chemo drugs on a cancer cell line. The graph compare the percentage of cancer cell growth inhibition and percentage of cancer cell killing...
12 July 2016 8,642 3 View
I plotted standard cancer cell growth curve for 24, 48, 72, 96 hrs. The cell concentration varies from 5000 to 50000 cells. For example, initially I seeded 10000 cells to 96 well plate. After...
11 July 2016 152 10 View
I am loading Gemcitabine HCl into mesoporous silica nanopariticles. I took 25 mg of mesoporous silica nanoparticles and 10 mg of drug for loading purpose. I used PBS 7.4 as a medium to load the...
01 January 1970 9,148 7 View
If we change the PH of the solution from 7.4 to 9, it will affect the drug loading percentage in nanoparticles.
01 January 1970 9,576 3 View
I am working on mesoporous silica nanoparticles for the treatment of pancreatic cancer. I am planning to put PEG over the surface of the mesoporous silica nanoparticles. I want good suggestions...
01 January 1970 2,132 3 View