I am synthesizing the plga nanoparticles with an anticancer drug. I took 100 microlitre of drug loaded plga nanoparticles and add 900 microlitre of acetone to it. Then I sonicate the drug loaded nanoparticles for 30 minutes to leach out the drug from polymer. I did centrifugation at 10000 rpm for 10 minutes to separate the polymer from drug. Then I did UV for the sample. I found that 43.3 microgram of drug was there in 100 microlitre of sample. Then I did same sample in HPLC. I found that 12.5 microgram of drug was there in 100 microlitre of sample. Why there is a big difference in drug loading % in two methods? I should consider which method??
If there is the possibility that something else absorbs light along with the drug, hplc is the answer. As long as i remember acetone dissolves plga.
Hi, I agree with the 2 answers above, but I recommend you to do the validation method of analysis first before doing any routine analysis. By doing validation you can decide which method is suitable or valid for your purpose of analysis. Without doing validation we can not know whether our method can be applied or not.