Pooja Singh

9 Questions 10 Answers 0 Followers

Questions related from Pooja Singh

How to check Beta Lactamase enzyme concentration and activity using Nitrocefin?

Dear All, Hope are you doing well. I am working on beta lactamase inhibitors. I have beta-lactamase-producing bacteria and i will have to check the concentration and enzyme activity for...

21 June 2023 7,461 3 View

Can we use any other substrate on the place of Keratin azure in keratinase enzyme assay?

Hi, I want to determine the enzyme activity of keratinase in the sample. I saw some literature regarding the same. where Keratin azure is mentioned as a substate for the same experiment. I do not...

19 April 2022 3,212 2 View

How to check whether endophytes have similar secondary metabolites to their host plant or not?

Hi, Everyone I want to check the secondary metabolites of endophytes whether endophytes have similar phytochemicals or not. I have lots of endophytes isolated from medicinal plants. Kindly suggest...

27 January 2022 647 4 View

What computer configurations are needed for the Autodock vina and Autodock docking software?

Hi, I downloaded Autodock (http://autodock.scripps.edu/downloads/autodock-4-2-x-installation-on-windows/) and Autodock vina (http://vina.scripps.edu/download.html) and installed but they are not...

11 May 2020 5,599 3 View

How to submit fungal sequence on NCBI?

Hi, can anyone tell me how to submit fungal sequence on NCBI? I isolated fungi from root of the host plant and made them sequence by 18s rDNA sequencing. I want to get accession number of fungus...

07 April 2020 9,130 4 View

How can i store whole plants for long time for staining purpose to view under confocal microscope?

I have to check endophytes inside plant sample to view under confocal microscope. So for the sample preparation, fresh sample will be collected and stored for further use. Can any one please tell...

14 August 2018 3,400 2 View

How to elute PCR product from agarose gel without any DNA purification kit?

Hi, I need to send my samples for sequencing before that i need to purify my samples. can anyone please tell me how do i purify my samples without using a DNA purification kit?

21 September 2017 1,733 7 View

What is difference between RFLP and T-RFLP?

Hi, can anyone tell me difference between RFLP and T-RFLP? i have to identify the endophyte from plant DNA samples then what should i prefer RFLP or T-RFLP?

28 April 2017 5,478 16 View

Total DNA extraction of endophytes (fungi and bacteria) from plant tissue

What is best method for total DNA extraction of endophytes from plant tissue? Can i prefer CTAB method for total DNA extraction or any DNA isolation kit? If kit is best then please suggest me kit...

14 April 2017 4,145 28 View