Hi all.
My lab is starting chemotherapy drug sensitivity assays for oral cancer cell lines. We will be detecting cell viability with the MTT and SRB assays, both of which are fluorometric. Hence, we'll need to purchase a fluorescence microplate reader.
Which brand's plate reader/product is the best? We are looking into affordable options that work for 96 well plates and ideally other formats as well. Is there one you guys are using in your lab that is good and efficient?
It would be really helpful if I could be guided into making the right purchase!
I worked in three labs using the Tecan safire 2, infinite F50(absorbance only) and Infinite 200 PRO.
Very good and reliable machines, but purchase cost is high.
Btw, MTT is a colorimectric assay if you're talking about the viability assay which gives you purple formazan (which absorbance is read out).
Dear Omama Syed
there are many good provider of fluorescence readers
eg. Tecan, Molecular device,
from my point of view the essential is that the instrument is not equiped only with filters but also with a monocromator, which allow you to free select any excitation/emission wavelenght that you mayneed in the future.
best regards
Manuele
- Badges
- Science topic
- Similar topics
- Engineering
- Modeling