We have been performing a thioflavin-T dose-response curve on alpha-synuclein fibrils with very consistent results for some time now.
Recently, we received a new batch of fibrils produced by the same method as before, but by a different person as the original person has moved elsewhere. Since then we have not been able to get the same dose-response curve.
If you look at the attached graph, curve E7 was obtained using the original fibres and curves E16 1 and E16 2 using the new batch. Does anyone have any idea what might be happening?
I have had a lot of help with this experiment on here, so I hope someone can help with this question too.
Previous discussions on this experiment are below.
https://www.researchgate.net/post/How_important_is_the_excitation_wavelength_for_alpha-synuclein_fluorescence_assay_with_thioflavin_T
https://www.researchgate.net/post/Which_96_well_plate_would_you_recommend_for_a_fluorescence_assay_with_thioflavin_T
I forgot to mention in the question that the concentration of the fibrils is kept constant and varying concentration of thioflavin-T is used to obtain the curve.
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