What is the ideal concentration of acridine orange solution should be used in fluorescent microscopy for bull sperm analysis?

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What is the best storage method for eluted samples for Infectious Diseases panels for Molecular PCR testing?

We run Fungal Panel using Molecular PCR Technique. We extract Nuclease Free water in the same way as we extract the sample. This eluted NFW is used as negative control on our PCR Plate for quality...

16 April 2024 776 1 View

How to do calculation for CVI and CVR in content validation based on likert scale?

In order to calculate CVI and CVR, the 4-points of Likert Scale frequently being adapted to determine the item validation. The indicators of 4-points used degree of relevance (1-not relevent,...

04 March 2024 2,838 2 View

Are there any recommended references in selecting experts before conducting pilot testing of questionnaires in service quality of public transport?

I am looking for references to look for the criteria of experts groups before conducting pilot testing. The experts i believe will receive the questionnaires to review before the real study being...

25 February 2024 1,519 3 View

Can anyone suggest why the U87-MG glioblastoma cell line did not attach after thawing?

The cells do not confluency after thawing. Even after 10 days, the color of the medium does not change. I change the medium, the non-adherent cells disappear and then the density in the flask...

11 December 2023 7,002 2 View

What is the most accepted guideline for Waste management plan for veterinary hospitals/ veterinary laboratories.?

what is the most (internationly) accepted waste management guideline for veterinary hospitals and veterinary laboratories for medical waste in veterinary hospitals and laboratories, how can we...

24 July 2023 2,006 3 View

What the reason PCR identification E.coli using usp A gene?

What makes usp A (universal stress protein) gene considered valid for molecular identification of Escherichia coli bacteria (used as PCR Primer)? Why use usp A while there are several groups of...

18 June 2023 4,022 2 View

How to dissolve plant ethanolic extract for seed germination assay?

How to dissolve plant ethanolic extract in sterile aquades if it is not dissolving completely? Should i dissolve it first using DMSO or ethanol in low conc before dilute it with aquades?

29 March 2023 2,769 1 View

Can someone suggest a good advanced reference Book of Public Finance for Economics?

Hi: I need any advanced Public Finance Reference Book for post-graduate studies. the reference with mathematical applications or methods. Regards

03 December 2022 4,396 5 View

We need to do a multivariate regression with two dependent variables and three independent variables. Can anybody suggest me which software to use?

We need to understand the relationship between two dependent variables (Frictional Noise and Coefficient of Friction) and three independent variables (Material Hardness, Surface Roughness, and...

12 July 2022 257 2 View

How to create an Ensemble of model outputs?

I have raster images from three models and I want to create their ensemble output. The correlation among rasters is near 70%. Is it a scientifically true approach? Can we combine 3 model outputs...

05 July 2022 5,345 3 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

What binders suggestion that I can use for zinc alloy?

I am working on powder metallurgy, and during the compaction process, the powder does not pelletize and remains in its powder form. To address this issue, I am considering using binders to improve...

13 July 2024 9,003 2 View

Are there different databases similar to the Zinc database or how can I solve this problem in the Zinc database?

We were using the Zinc database for the virtual compound library in our studies, but there have been problems downloading a large number of compounds for a while. Are there different databases...

30 June 2024 8,144 1 View

Which free software is preferable for DFT calculations?

I want to perform DFT calculations for a triple cation mixed crystal. Which software is preferable and free?

28 June 2024 5,255 2 View

How to do DFT calculations and which free software is easy recommended for that?

i want to do DFT calculations for a triple cation mixed crystal. Can somebody help me in this regard?

27 June 2024 1,804 0 View

What is the most accurate way to determine the energy gap of semiconductor materials? Is it better to use UV-Vis or PL measurements?

When preparing hierarchical zinc oxide ananostructure, the energy gap measured by PL was equal to (3.26 eV) While the energy gap value was determined by UV-Vis measurements (3.5 to 3.7 eV)

26 June 2024 7,505 6 View