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Questions related from Nikhil Goel
Hello, I need to take the sodium nucleotide salts I have available in my lab and modify them to instead contain some more exotic counterions. Can I do this by binding my nucleotides to a strong...
09 January 2023 3,964 3 View
Hello! I was wondering if I would need to break the disulfide in my compound and then protect the thiols before performing michael addition reaction on primary and seconadry amines? Planning on...
31 August 2022 5,134 0 View
Im wondering if anyone can point me to any literature discussing the benefits of the AAV2 ITR in manufacturing, therapeutic efficacy, clearance, toxicity, etc. Not sure why everyone has chosen to...
27 August 2021 2,299 1 View
Hi All, Ive been constructing some plasmids with 4-12 pieces at a time pretty regularly using golden gate assembly for about 6 months now, but its getting to be a bit cumbersome due to relatively...
09 March 2021 7,282 2 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
02 March 2021 9,103 3 View
Im trying to optimize my downstream process for AAV processing and I've been having quite a bit of trouble for some reason, I described my process below: I lysed cells in production media with...
01 December 2020 1,582 1 View
Spcas9 has been reported to cleave ~ 3bp upstream of the start of the PAM as a blunt cut, Cpf1 has a staggered cut significantly upstream of the PAM, but I cant seem to find much information...
30 September 2020 9,077 2 View
I cant seem to find any good structural papers talking about the exact residues of each of VP1, 2, and 3 that are solvent exposed. From what I've read it sounds like VP1 and VP3 are both...
21 September 2020 8,292 2 View
I may have misunderstood something but I thought that all homologous recombination events depend on an initial step of MRN/ATM. Many Ad vectors are Ad5 based which contains an E4Orf3 which...
21 June 2020 4,993 1 View
I would like to crosslink 10 bp amine terminated DNA probes onto carboxylated microspheres at a controlled density. The carboxyl density on my beads is 2-7 (A^2/carboxyl), however I want to have a...
29 August 2019 6,281 2 View
I am about to build an electrospinning system using >20kV and I want to make sure that I will be able to operate this thing safely. Someone mentioned that to discharge this, I would need to...
17 April 2018 975 1 View
Or what is the smallest known fluorescent molecule?
14 December 2017 7,620 3 View
Are there bacteria or fungi with immune systems to attack neighboring foreign cells? Do insects exhibit GVHD (can they distinguish their own cells from another insect of the same species)?
12 September 2017 3,278 7 View
I would like to perform an experiment that is dependent on ssDNA successfully flowing out of a PDMS microchannel for collection. However, ssDNA adsorbs onto hydrophobic surfaces, and PDMS is...
24 August 2017 5,973 0 View
I need to functionalize a glass surface with DNA (my method of functionalization is crosslinking amine terminated DNA to aminosilanized glass with gluteraldehyde). I then need to bond that...
04 August 2017 6,918 4 View
Anyone know of any papers that discuss the size of different aptamers with respect to their target? Or papers that have created anti- antibody aptamers?
05 July 2017 1,875 0 View
I remember hearing somewhere that the FDA does not allow the supplementation of tetracycline. And by good I mean little to no expression before induction and an inducing agent that will not...
06 September 2015 1,020 0 View
Im thinking about doing some work with >80kb plasmids and I wanted to get a better understanding for working with such large vectors. It seems like BAC is the only way to go and pBeloBAC11 is...
01 January 1970 5,959 3 View
Im new to data science, and was wondering if someone could point me in the right direction to start understanding how to perform the correct statistical analysis/decomposition/modeling analysis of...
01 January 1970 5,162 4 View
Looking to see if anyone can spare a few vials of an immortalized adipocyte cell line. Doesn't really matter if its white, brown or beige or if its preadipo or adipo. hoping for human but mouse or...
01 January 1970 6,363 0 View
