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Questions related from Nikhil Goel
Hello, I need to take the sodium nucleotide salts I have available in my lab and modify them to instead contain some more exotic counterions. Can I do this by binding my nucleotides to a strong...
09 January 2023 3,952 3 View
Hello! I was wondering if I would need to break the disulfide in my compound and then protect the thiols before performing michael addition reaction on primary and seconadry amines? Planning on...
31 August 2022 5,121 0 View
Hi All, Ive been constructing some plasmids with 4-12 pieces at a time pretty regularly using golden gate assembly for about 6 months now, but its getting to be a bit cumbersome due to relatively...
09 March 2021 7,271 2 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
02 March 2021 9,047 3 View
Im trying to optimize my downstream process for AAV processing and I've been having quite a bit of trouble for some reason, I described my process below: I lysed cells in production media with...
01 December 2020 1,557 1 View
Spcas9 has been reported to cleave ~ 3bp upstream of the start of the PAM as a blunt cut, Cpf1 has a staggered cut significantly upstream of the PAM, but I cant seem to find much information...
30 September 2020 9,066 2 View
I cant seem to find any good structural papers talking about the exact residues of each of VP1, 2, and 3 that are solvent exposed. From what I've read it sounds like VP1 and VP3 are both...
21 September 2020 8,283 2 View
I am about to build an electrospinning system using >20kV and I want to make sure that I will be able to operate this thing safely. Someone mentioned that to discharge this, I would need to...
17 April 2018 966 1 View
Or what is the smallest known fluorescent molecule?
14 December 2017 7,605 3 View
Are there bacteria or fungi with immune systems to attack neighboring foreign cells? Do insects exhibit GVHD (can they distinguish their own cells from another insect of the same species)?
12 September 2017 3,266 7 View
Im thinking about doing some work with >80kb plasmids and I wanted to get a better understanding for working with such large vectors. It seems like BAC is the only way to go and pBeloBAC11 is...
01 January 1970 5,947 3 View
Im new to data science, and was wondering if someone could point me in the right direction to start understanding how to perform the correct statistical analysis/decomposition/modeling analysis of...
01 January 1970 5,147 4 View
