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Questions related from Nazmul Islam
I used 0.5 ug plasmid. Cell confluency around 60%. Transfection time is 48 hour. I used empty plasmid (pCMV-3Tag-4A) and only Lipofectamine as a negative control. However, I used EGFP plasmid tag...
04 August 2023 1,842 2 View
I am using Optineurin gene and I want to establish stable cell line for specific gene expression. But when I have transfection using antibiotic resistance media, it almost died nearly 90%. SO...
05 November 2022 4,564 6 View
In details before sectioning I fix the one set of spleen sample in 10% buffered formaldehyde 72h As well as another set in 4% para formaldehyde in 24 h.But while sectioning I am facing the same...
02 July 2021 9,253 6 View
I have a problem using the Drp1 C5 and p-Drp1 S616 antibodies from Santa Cruz for the western blotting technique. The band should be at around 70-80kD, so I used 10% gel. I used to transfer 90 V...
27 June 2021 9,433 6 View
My bacteria sample is Acinetobacter baumannii. I found one protocol just centrifuge and using congo red and check OD 490 nm supernatant. I need more better protocol for extraction EPS with reference.
27 May 2020 9,837 2 View
Actually I work in bacteriophage resistance mechanism. So I found my bacteria look like different structure show in normal nutrient ( 1x LB) and lower nutrient (0.25x LB). When i used bacteria...
28 April 2020 671 0 View
For capsule staining I used over night bacteria culture and smear with 1% congo red . After that, dry the slide in room temperature and observe under light microscopy 100x magnification oil...
18 February 2020 7,508 8 View
I used India Ink and 1% CV for Identification CPS layer in oil immersion Light microscopy (100x). My bacteria sample is Acinetobacter baumannii .
02 February 2020 9,578 3 View
For OMV isolation, I used overnight bacteria culture and centrifuge 10,000 g for 15 min then supernatant 0.22 um filter and then filters 12 ml ultracentrifuge 38,000 g 3 hours 4 degree. After then...
14 November 2019 9,354 2 View
I used PBS for washed pellet. Then put into Ice for 1 hour with phage MOI 10. Then negative staining.
27 September 2019 7,724 2 View
Capsule, staining, motility, bacteria, Capsule identification, Assays, confocal microscopy.
13 September 2019 7,965 1 View
Biofilm Formation Biofilm Acinetobacter baumannii Microbial Biofilms Bacterial Biofilm
14 November 2018 3,875 4 View
I am working in different kind of cell death pathway and working macrophage cell line so my aim is to find out different kind of cell death. So any one can explain why it happened ?
01 January 1970 4,952 3 View
