Nazmul Islam

11 Questions 29 Answers 0 Followers

Questions related from Nazmul Islam

Why is transfection efficiency low when using Lipofectami™ 3000 Transfection Reagent with WT Hella cells for transient transfection?

I used 0.5 ug plasmid. Cell confluency around 60%. Transfection time is 48 hour. I used empty plasmid (pCMV-3Tag-4A) and only Lipofectamine as a negative control. However, I used EGFP plasmid tag...

04 August 2023 1,832 2 View

Why my transfection efficacy is low where I using Lipofectamine™ 3000 Transfection Reagent in Hela cells ?

I am using Optineurin gene and I want to establish stable cell line for specific gene expression. But when I have transfection using antibiotic resistance media, it almost died nearly 90%. SO...

05 November 2022 4,554 6 View

Why my spleen sample getting broken while sectioning ?? What types of problem here occured ?

In details before sectioning I fix the one set of spleen sample in 10% buffered formaldehyde 72h As well as another set in 4% para formaldehyde in 24 h.But while sectioning I am facing the same...

02 July 2021 9,240 6 View

Can any one give me the best protocol of EPS extraction and quantification from gram negative bacteria ?

My bacteria sample is Acinetobacter baumannii. I found one protocol just centrifuge and using congo red and check OD 490 nm supernatant. I need more better protocol for extraction EPS with reference.

27 May 2020 9,824 2 View

Can any one find look like this structure in TEM image for bacteria ?

Actually I work in bacteriophage resistance mechanism. So I found my bacteria look like different structure show in normal nutrient ( 1x LB) and lower nutrient (0.25x LB). When i used bacteria...

28 April 2020 656 0 View

Can any one used congo red for capsule staining for gram negative bacteria Acinetobacter baumannii ?

For capsule staining I used over night bacteria culture and smear with 1% congo red . After that, dry the slide in room temperature and observe under light microscopy 100x magnification oil...

18 February 2020 7,491 8 View

Can anyone give me suggestion how can I get more better picture for capsular polysaccharide from bacteria sample in Light microscopy ?

I used India Ink and 1% CV for Identification CPS layer in oil immersion Light microscopy (100x). My bacteria sample is Acinetobacter baumannii .

02 February 2020 9,558 3 View

Can anyone give me the best protocol of Purification & quantification of capsule polysaccharide & outer membrane vesicle from Acinetobacter baumannii?

For OMV isolation, I used overnight bacteria culture and centrifuge 10,000 g for 15 min then supernatant 0.22 um filter and then filters 12 ml ultracentrifuge 38,000 g 3 hours 4 degree. After then...

14 November 2019 9,341 2 View

Why it is not clear background after treated bacteria with phage in TEM analysis ? Can any one give me protocol of TEM for phage treat with bacteria ?

I used PBS for washed pellet. Then put into Ice for 1 hour with phage MOI 10. Then negative staining.

27 September 2019 7,708 2 View

Can any one give me the protocol of Capsule staining of bacteria and motility assay of bacteria ?

Capsule, staining, motility, bacteria, Capsule identification, Assays, confocal microscopy.

13 September 2019 7,951 1 View

Why multiple cell death initiated in macrophage ?

I am working in different kind of cell death pathway and working macrophage cell line so my aim is to find out different kind of cell death. So any one can explain why it happened ?

01 January 1970 4,935 3 View