Can any one give me the best protocol of EPS extraction and quantification from gram negative bacteria ?

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Khajezade Mohadese

please tell the kind of your EPS

for example xhantan extracted with alcohol

Aparna Banerjee

For extraction, you can simply centrifuge & precipitate with ethanol/ acetone. Additionally, you may partially purify it with TCA precipitation to take out the residual proteins. For quantification, you may use Congo red, apart from that alcian blue is also an option.