What are my options to precipate out protein eluted from Ni-NTA Column?

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Sudhakar Srivastava

If you want to concentrate your protein, you can use spin columns that comes with different cut off value based on the size of proteins. It will not only help you to concentrate the protein, but it will additionally purify it and also you can change the buffer, which you would require for your subsequent experiments.

Naveen Sathyan

Thank you all for responding. I would definitely try your suggestions.