Nandita Ghosh

7 Questions 1 Answers 0 Followers

Questions related from Nandita Ghosh

What can be the possible solvent to get monomers of peptides that contain mostly hydrophobic residues?

Usually, people working with short as well as long peptide sequences first dissolve it in either HFIP or DMSO to make sure that all the molecules are in monomeric form. But for my short synthetic...

21 November 2023 4,339 1 View

Can anyone suggest about how to perform a turbidity experiment with a peptide showing liquid-liquid phase separation property?

One of our synthesized peptide shows phase separation behavior at a certain physiological condition. I mean to ask what can be the exact protocol that we should follow for the turbidity experiment.

14 August 2023 3,132 0 View

What can be the probable precautions to be taken and cleavage cocktail to be used in synthesis of Trp containing peptide?

I have a target heptad sequence containing amino acids Aib, Phe, Ala and Trp at different positions. What can be the standard protocol to follow while coupling the Trp amino acid and the cleavage...

31 July 2023 3,661 0 View

What are the precautions and preventions that should be maintained to handle 2-chlorotritylchloride resin?

What is the best coupling procedure and conditions to be followed to attach the C-terminal amino acid, Aib (2-aminoisobutyric acid), into a 2-chlorotrityl chloride resin? How to maintain the inert...

28 September 2022 8,096 0 View

What can be the cleavage cocktail used in synthesis of Trp-Trp-Phe tripeptide?

I have synthesized a tripeptide Trp-Trp-Phe using Fmoc-Phe-Wang resin and Boc protected Fmoc-Trp amino acid with the coupling reagents HOBt/HBTU/DIPEA. After all the coupling process the global...

20 September 2022 4,530 1 View

Can anyone help me out regarding the cleavage procedure of a heptad peptide sequence on Rink amide resin?

I have synthesized a seven amino acid sequence having a C-terminal amide Phe-Aib-Leu-Ala-Aib-Leu-Phe-NH2 by Rink amide resin. I have got the mass spectrometry done and somehow the peptide didn't...

20 June 2022 1,740 0 View

What resin to be used and what variety of the Cysteine amino acid to be used?

Leu-Aib-Phe-Cys-Leu-Aib-Phe is my target peptide to be synthesized by solid phase peptide synthesis. Since Cysteine is a oxidation prone amino acid and Phe is in the C-terminal amino acid, can...

27 March 2022 4,272 2 View