27 June 2021 2 4K Report

What is the best method of labelling extracellular vesicles for in vitro experiences?

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Hey, does anyone know any effective way to separate virus from exosomes fraction? my problem with the separation of the EVs in this system is the fact that the virus that we work with particles...

06 December 2020 4,765 2 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

03 December 2020 6,488 2 View

What are the XRD analysis conditions of the cement, sand and Limestone ?

I am a Phd student and I need to do XRD analysis on my pouder samples namely ( cement- sand- limestone and calcined clay). I was asked asked to specify the analysis conditions: -the nature of...

07 October 2020 5,737 6 View

Can anyone recommend me some articles about Fire endurance of wood concrete?

Are there documentations about Fire endurance of wood or wood shavings concrete? The literature is not very clear about this .

12 August 2020 8,203 3 View

Is it possible to use plant extract as natural sterilizer?

For economic reasons, sterilzation against covid_19 is very necessary, so is it possible to use plant extract?

11 May 2020 2,674 14 View

Which statistical analysis is more appropriate for my study?

I am trying to find the relationship between the use of defence mechanisms in early adulthood as predictors of neuroticism. Those who use more defence mechanisms will score higher on the...

14 April 2020 3,353 7 View

How do I carry out performance evaluation on a sorting machine?

I design a prototype for sorting tomatoes based on size and colours. The prototype will sort big red, big green, small green and small red

12 March 2020 2,386 3 View

How can i characterise the crud extract of peganum harmala seeds ?

Hello everyone, The crud extracts of peganum harmala seeds contains alkaloids and polyphenolic compounds and i want to do the HPLC , LC-MS or RMN without extracting them, can you give me the...

06 March 2020 6,849 4 View

Preparation of the mix for Standard PCR (without using a kit)?

Hi, Please anyone already prepared the mix for Standard PCR? But without using a commercial kit. It is for staphylococcal enterotoxins genes. I am looking for the concentrations of each...

08 February 2020 4,487 6 View

How to deal with mental health issues?

I feel communication helps in coping such issues to some level.

16 August 2019 7,405 31 View

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Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

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28 February 2021 3,442 2 View

RIA. How to measure?

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28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

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