Muhammad Rizwan

11 Questions 10 Answers 0 Followers

Questions related from Muhammad Rizwan

What conditions are required to react isocyanate with COOH or OH groups?

I would like to react isocynate (NCO) to COOH or OH groups in DMSO environment. Do I need to activate the COOH or OH groups with carbonyldiimidazole? or do I need to use catalyst suchas...

05 December 2019 7,561 4 View

Can we use platelet rich plasma (PRP) obtained from heparinized blood for in-vitro thrombin generation assays?

We collect blood by using heparin as anticoagulant instead of citrate. I want to characterize the thrombin generation potential of the PRP after our experiment. People have usually used citrated...

17 November 2017 4,855 3 View

How can we analyze the products of bio-degradation of gelatin-based hydrogel?

The idea is to investigate if there are 'unwanted' compounds formed as a result of bio-degradation.

07 May 2017 5,625 3 View

How do you create a multi-color cell migration video in ImageJ?

I know that we can import image sequence (images taken at different times of cell migration) and convert it into video files. However, this only works for one type of image sequence, such as phase...

10 March 2015 5,058 5 View

Is it possible to graft amine or methacrylate groups to Graphene Oxide?

For amine conjugation: Would EDC chemistry work given that the GO has carboxylic groups? For methacrylation: Is it possible to methacrylate the OH groups of GO? How easy or difficult are the above...

19 November 2014 5,897 4 View

What is the strongest (in terms of compressive/tesile strength) biopoymer based hydrogel?

It could be an IPN/composite hydrogel of two bio-polymers as well as for example gelatin and silk. Strength should be reported when it is in hydrated form and not dry strength. Please also provide...

20 November 2013 812 2 View

How do you do localized (in the center only) cell seeding on your samples?

I have 1cmx1cm TCPS samples and I only want to seed cells in the center (~0.3cm2 area). I have tried making PDMS rings and gluing it to the samples but there is always a leakage issue. If I do not...

24 September 2013 9,698 5 View

Why do we need to neutralize the collagen solution if we want to make a collagen hydrogel film? What are the swelling ratios of collagen hydrogel?

I am new to collagen gel and tried to look for protocols for collagen gel film making. Briefly, the collagen solution (in acetic acid) is neutralized and then left at 37 degree for about 30-60 min...

10 March 2013 9,370 8 View

Will a UV-crosslinked hydrogel collapse during dehydration in air?

I was working with a methacrylated gelatin hydrogel that was cross linked by UV exposure. After cross linking, I put the gel for a few hours and I noticed that It got dehydrated and I got a thin...

13 February 2013 9,512 8 View

Where can we purchase "medical-grade" gelatin (or collagen for that matter)?

I am looking for medical-grade gelatin. Actually our hydrogel scaffold (Gelatin) may ultimately be transplanted in human. Is there any thing like medical grade and where can we purchase it?

18 January 2013 6,841 9 View

How do you purify hydrogels for cell seeding?

I am preparing gelatin hydrogel and need to purify it after its methacrylation. I'll use that as a substrate for cell seeding so I want to remove any unreacted components from the gelatin...

08 January 2013 8,613 2 View