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Questions related from Muhammad Rizwan
I would like to react isocynate (NCO) to COOH or OH groups in DMSO environment. Do I need to activate the COOH or OH groups with carbonyldiimidazole? or do I need to use catalyst suchas...
05 December 2019 7,518 4 View
We collect blood by using heparin as anticoagulant instead of citrate. I want to characterize the thrombin generation potential of the PRP after our experiment. People have usually used citrated...
17 November 2017 4,818 3 View
For amine conjugation: Would EDC chemistry work given that the GO has carboxylic groups? For methacrylation: Is it possible to methacrylate the OH groups of GO? How easy or difficult are the above...
19 November 2014 5,857 4 View
It could be an IPN/composite hydrogel of two bio-polymers as well as for example gelatin and silk. Strength should be reported when it is in hydrated form and not dry strength. Please also provide...
20 November 2013 766 2 View
I have 1cmx1cm TCPS samples and I only want to seed cells in the center (~0.3cm2 area). I have tried making PDMS rings and gluing it to the samples but there is always a leakage issue. If I do not...
24 September 2013 9,659 5 View
I am new to collagen gel and tried to look for protocols for collagen gel film making. Briefly, the collagen solution (in acetic acid) is neutralized and then left at 37 degree for about 30-60 min...
10 March 2013 9,334 8 View
I was working with a methacrylated gelatin hydrogel that was cross linked by UV exposure. After cross linking, I put the gel for a few hours and I noticed that It got dehydrated and I got a thin...
13 February 2013 9,476 8 View
I am looking for medical-grade gelatin. Actually our hydrogel scaffold (Gelatin) may ultimately be transplanted in human. Is there any thing like medical grade and where can we purchase it?
18 January 2013 6,797 9 View
I am preparing gelatin hydrogel and need to purify it after its methacrylation. I'll use that as a substrate for cell seeding so I want to remove any unreacted components from the gelatin...
08 January 2013 8,576 2 View