Muhammad Rizwan

6 Questions 6 Answers 0 Followers

Questions related from Muhammad Rizwan

Can we use platelet rich plasma (PRP) obtained from heparinized blood for in-vitro thrombin generation assays?

We collect blood by using heparin as anticoagulant instead of citrate. I want to characterize the thrombin generation potential of the PRP after our experiment. People have usually used citrated...

17 November 2017 4,810 3 View

Is it possible to graft amine or methacrylate groups to Graphene Oxide?

For amine conjugation: Would EDC chemistry work given that the GO has carboxylic groups? For methacrylation: Is it possible to methacrylate the OH groups of GO? How easy or difficult are the above...

19 November 2014 5,848 4 View

What is the strongest (in terms of compressive/tesile strength) biopoymer based hydrogel?

It could be an IPN/composite hydrogel of two bio-polymers as well as for example gelatin and silk. Strength should be reported when it is in hydrated form and not dry strength. Please also provide...

20 November 2013 760 2 View

How do you do localized (in the center only) cell seeding on your samples?

I have 1cmx1cm TCPS samples and I only want to seed cells in the center (~0.3cm2 area). I have tried making PDMS rings and gluing it to the samples but there is always a leakage issue. If I do not...

24 September 2013 9,652 5 View

Why do we need to neutralize the collagen solution if we want to make a collagen hydrogel film? What are the swelling ratios of collagen hydrogel?

I am new to collagen gel and tried to look for protocols for collagen gel film making. Briefly, the collagen solution (in acetic acid) is neutralized and then left at 37 degree for about 30-60 min...

10 March 2013 9,327 8 View

How do you purify hydrogels for cell seeding?

I am preparing gelatin hydrogel and need to purify it after its methacrylation. I'll use that as a substrate for cell seeding so I want to remove any unreacted components from the gelatin...

08 January 2013 8,567 2 View